Synaptic activity-dependent regulation of microtubule transport in the delivery of plasticity-related mRNAs and proteins

可塑性相关 mRNA 和蛋白质传递过程中微管运输的突触活动依赖性调节

• 批准号: 282800777
• 负责人: Professor Dr. Matthias Kneussel, Ph.D.
• 金额: --
• 依托单位: Zentrum für Molekulare Neurobiologie (ZMNH)
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/282800777?language=en
• 关键词: Synaptic; activity; dependent; regulation; microtubule

We investigate the interrelationship between synapse structure, synapse function and microtubule (MT) transport. Plasticity-related products (PrPs), such as synaptic mRNAs and proteins are transported along a dynamic MT cytoskeleton. We apply genetic, molecular, physiological and optogenetic approaches to ask how synaptic activity regulates MT dynamics, MT posttranslational modifications and the binding of MT-associated proteins (MAPs). By manipulating these cytoskeletal parameters, we investigate changes in synaptic mRNA and protein delivery. We aim to understand whether neuronal MT-subtypes encode an activity-dependent signature to promote certain transport processes over others.

我们研究了突触结构、突触功能和微管运输之间的相互关系。可塑性相关产物(PRPS)，如突触的mRNAs和蛋白质，沿着一个动态的MT细胞骨架运输。我们运用遗传学、分子、生理学和光遗传学的方法来研究突触活动如何调节MT的动态、MT的翻译后修饰和MT相关蛋白(MAP)的结合。通过操纵这些细胞骨架参数，我们研究了突触mRNA和蛋白质递送的变化。我们的目标是了解神经元MT亚型是否编码依赖于活动的信号来促进某些运输过程。