Synaptic activity-dependent regulation of microtubule transport in the delivery of plasticity-related mRNAs and proteins

我们研究了突触结构、突触功能和微管运输之间的相互关系。可塑性相关产物(PRPS)，如突触的mRNAs和蛋白质，沿着一个动态的MT细胞骨架运输。我们运用遗传学、分子、生理学和光遗传学的方法来研究突触活动如何调节MT的动态、MT的翻译后修饰和MT相关蛋白(MAP)的结合。通过操纵这些细胞骨架参数，我们研究了突触mRNA和蛋白质递送的变化。我们的目标是了解神经元MT亚型是否编码依赖于活动的信号来促进某些运输过程。

We investigate the interrelationship between synapse structure, synapse function and microtubule (MT) transport. Plasticity-related products (PrPs), such as synaptic mRNAs and proteins are transported along a dynamic MT cytoskeleton. We apply genetic, molecular, physiological and optogenetic approaches to ask how synaptic activity regulates MT dynamics, MT posttranslational modifications and the binding of MT-associated proteins (MAPs). By manipulating these cytoskeletal parameters, we investigate changes in synaptic mRNA and protein delivery. We aim to understand whether neuronal MT-subtypes encode an activity-dependent signature to promote certain transport processes over others.