Engineering of biofunctionalized vascular grafts using elektrospinning of human extracellular matrix and additional oligonucleotid coating for capturing of circulating endothelial progenitor cells (EPC)

使用静电纺丝人类细胞外基质和额外的寡核苷酸涂层来捕获循环内皮祖细胞（EPC）来工程化生物功能化血管移植物

基本信息

批准号：
285646607
负责人：
Professor Dr. Andres Beiras Fernandez
金额：
--
依托单位：
Klinik und Poliklinik für Herz- und Gefäßchirurgie
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2015
资助国家：
德国
起止时间：
2014-12-31 至 2022-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/285646607?language=en
关键词：
Engineering biofunctionalized vascular grafts using

项目摘要

The current treatment of aneurysatic or stenpotic large diameter vascular grafts uses two different prosthetic materials. Dacron prothesis serve the purpose of a blood vessels with good hemocompatibility however have the disadvantages of a constant infection risk, an inability to grow or remodell and pseudointimal peeling. Alternatively used allogenic vascular grafts reduce the risk for peeling and infection and are accordingly broadly used for replacement of infected prosthetic vascular grafts. Beside a limited availability due to organ scarcity these allografts tend to calcify heavily in particular in the pediatric population. Optimal arterial graft materials consist of human tissue components with characteristics of native arteries including an endothelial cell lining. Autologous endothelial lining of PTFE-prosthesis showed successfull short- and mid-term results when used for peripheral vascular and coronary artery bypass grafting. The rather complex in vitro manufacturing is however accompanied with an infection risk. Objective of the grant proposal is the engineering of an off-the-shelf vascuar graft using elektrospinning of human hybrid extracellular matruix components (ECM) with an additional surface biofunctionalization using oliginucleotids in order to attract and bind circulating endothelial progenitor cells for an autologous in vivo endothelialization after implantation.

当前对动脉肿瘤或狭窄的大直径血管移植物的处理使用了两种不同的假肢材料。 DACRON原理的目的是具有良好血管的血管具有良好的血管，其缺点是持续的感染风险，无法生长，改造和假性脱皮。或者，使用的同种异性血管移植物降低了剥离和感染的风险，因此广泛用于替代感染的假肢血管移植物。除了因器官稀缺而导致的有限可用性之外，这些同种异体移植物倾向于在小儿种群中特别钙化。最佳动脉移植物材料由人体组织成分组成，其特征是天然动脉的特征，包括内皮细胞衬里。当用于周围血管和冠状动脉搭桥术时，PTFE生育的自体内皮内皮衬里显示出成功的短期和中期结果。然而，相当复杂的体外制造伴随着感染风险。赠款提案的目的是使用人类杂交杂交细胞外矩阵组件（ECM）进行搁置的Vascuar移植物进行工程，并使用寡核苷酸进行了额外的表面生物官能化，以吸引和结合自动性的内皮祖细胞，以吸引和结合循环循环的内皮细胞。