EFRI-CBE Topic B: Biofunctionalized Devices - On Chip Signaling and "Rewiring" Bacterial Cell-Cell Communication

EFRI-CBE 主题 B：生物功能化器件 - 片上信号传导和“重新布线”细菌细胞间通信

• 批准号: 0735987
• 负责人: William Bentley
• 金额: --
• 依托单位: University of Maryland Biotechnology Institute
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-10-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0735987&HistoricalAwards=false
• 关键词: EFRI; CBE; Topic; Biofunctionalized; Devices

PI name: W.E. BentleyInstitution: University of Maryland Biotechnology InstituteProposal Number: 0735987EFRI-CBE: Biofunctionalized Devices On Chip Signaling and Rewiring Bacterial Cell-Cell CommunicationAbstractThis project is to demonstrate signal translation by employing device-based electrical signals to guide the assembly of biosynthetic pathways, cell-based sensors, and cell-based actuators within a microelectromechnical system (MEMS), and to use on-board electrical, magnetic, mechanical, and optical systems to feedback and guide the cell-based system towards user-specified outcomes. The target of this project is the cell-cell communication system mediated by bacterial signaling autoinducers in a process known as quorum sensing. The Principal Investigators (PIs) have created a computational model that captures the dynamics of quorum signal generation, receptor driven recognition, and uptake. This model, based on biochemical and biophysical processes, will guide the conceptual design of subsystem synthons assembled architectures that guide heterologous protein synthesis in response to specific biomolecular cues. Cells will be signaled to initiate biofilm formation and maturation. The MEMS environment will enable for the first time, an experimental platform for the design, construction, and testing of this cell-based signal transduction process. Moreover, this MEMS environment will detect cell function and, by guiding signaling pathways, change cell phenotype in a controlled and directed manner.Engaged students will pursue both fundamental questions and technological goals within a multi-disciplinary environment that encourages diversity and fosters cooperation. Students will learn sciences that range from molecular and cell biology to signal processing and be exposed to issues of device fabrication and use. The PIs will enlist guidance and support from industry. This research may spawn new efforts on device fabrication, embedded sensor systems, bacterial pathogenicity, biofilm formation, genetic regulation and signal transduction. Developments are envisioned that impact fields of medicine (drug discovery, synthesis, and delivery), communications (biofunctionalized microfabricated devices), and security (smart sensors).

PI名称：W.E. BentleyInstitution：马里兰大学生物技术机构机构编号：0735987EFRI-CBE：芯片信号上的生物功能化设备，并重新布线细菌细胞细胞细胞网络通讯communication abrowthis communication turthis project in Project in Deproject this Project旨在通过启用基于设备的电信机来指导基于设备的电信机，以指导基于电池的电池，以指导基于小组的pathers pathers基于Biosnthe的pathersembly of Biosnththe conterly of Biosnththe，并在基于生物系统的组件中，并证明微电工系统（MEMS），并使用板载电气，磁性，机械和光学系统来反馈和指导基于细胞的系统，以实现用户指定的结果。该项目的目标是通过细菌信号传导自动诱导仪在被称为Quorum Sensing的过程中介导的细胞电池通信系统。主要研究人员（PIS）创建了一个计算模型，该模型捕获了法定信号产生，受体驱动识别和吸收的动力学。该模型基于生化和生物物理过程，将指导子系统合成子组装的概念设计，这些结构组装了构造，以指导异源蛋白质合成，以响应特定的生物分子提示。细胞将发出信号以引发生物膜形成和成熟。 MEMS环境将首次启用，这是一个实验平台，用于设计，构建和测试此基于细胞的信号传输过程。此外，此MEMS环境将检测细胞功能，并通过指导信号通路，以受控和指示的方式改变细胞表型。参与的学生将在鼓励多样性和促进合作的多学科环境中追求基本问题和技术目标。学生将学习从分子和细胞生物学到信号处理的科学，并面临设备制造和使用问题。 PI将获得行业的指导和支持。这项研究可能会产生有关设备制造，嵌入式传感器系统，细菌致病性，生物膜形成，遗传调节和信号转导的新工作。设想的发展是，影响医学的领域（药物发现，合成和交付），通信（生物功能化的微型制造设备）和安全性（智能传感器）。