Initial steps of thylakoid membrane formation and the role of endomembrane system-mediated protein targeting into plastids (B10)

类囊体膜形成的初始步骤以及内膜系统介导的蛋白质靶向质体的作用 (B10)

• 批准号: 289580743
• 金额: --
• 依托单位: Institut für Entwicklungs- und Molekularbiologie der Pflanzen
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/289580743?language=en
• 关键词: Initial; steps; thylakoid; membrane; formation

Project B10 aims to identify components and fundamental steps required for the formation of the highly organized thylakoid network in higher plant chloroplasts. One central aspect is to investigate the contribution of the endomembrane system to thylakoid formation by exploiting the previously identified thylakoid biogenesis factor HCF222. Our data show that this protein is targeted to chloroplasts via the endomembrane system-mediated pathway. Using a genetic approach, we intend to identify specific components and requirements involved in this non-canonical import pathway and its impact on thylakoid development. Moreover, we will use reverse genetic approaches to isolate novel proteins involved in early steps of thylakoid membrane formation, focusing on processes at the inner envelope.

B10项目旨在确定高等植物叶绿体中高度组织化的类囊体网络形成所需的成分和基本步骤。一个中心方面是通过利用先前鉴定的类囊体生物发生因子HCF222来研究膜系统对类囊体形成的贡献。我们的数据表明，该蛋白通过膜系统介导的途径靶向叶绿体。使用遗传方法，我们打算确定这种非规范进口途径中涉及的特定成分和要求及其对类囊体发育的影响。此外，我们将使用反向遗传方法分离参与类囊体膜形成早期步骤的新蛋白质，重点关注内层包膜的过程。