Assessment and modeling of bacterial regrowth after wastewater disinfection

废水消毒后的细菌再生长对公众健康构成严重威胁。本研究的目的是评估和模拟大肠杆菌（E。大肠杆菌）在氯化后的环境水中再生长。建立了一种基于荧光的活大肠杆菌定量方法。大肠杆菌在河水和污水处理厂的发展。平板计数和荧光法的多重检测表明，从活的但不可培养的状态的再激活是主要的再生长过程。再生模型表明，再生率和再生率受初始氯浓度和投氯量的限制，并受水体类型的影响。这项研究提出了一种新的方法，多重检测评估后氯化再生，有助于废水管理。

Bacterial regrowth after wastewater disinfection poses a severe risk to public health. This study aimed to assess and model Escherichia coli (E. coli) regrowth in environmental water after chlorination. A fluorescence-based method for quantifying viable E. coli in river water and treated wastewater was developed. The multiplex detection of plate count and fluorescence method suggested that the reactivation from the viable but non-culturable state was the main regrowth process. The regrowth model revealed that the regrowth rate and percentage were limited by increasing initial chlorine concentration and chlorine dose, and effected by the water type. This study presents a new approach with multiplex detection for assessing post-chlorination regrowth and contributes to wastewater management.