Analysis of chemicals and microorganisms functioning as the key in the suppression of Monochoria vaginalis by rice bran.

分析米糠抑制鸭舌草的关键化学物质和微生物。

• 批准号: 21K05552
• 负责人: 田澤 純子
• 金额: $ 2.58万
• 依托单位: National Agriculture and Food Research Organization
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21K05552/
• 关键词: 水稲; 有機栽培; 米ぬか散布; Clostridium属細菌; 有機酸; コナギ; 米ぬか; 水稲有機栽培; 嫌気性細菌

1）長期有機水稲栽培を継続した水田土壌を熱処理し、嫌気条件下の平板希釈法で得られたコロニーから、培養実験系でコナギ発芽阻害活性を示す2株を単離した。これらについて全ゲノム解析を行ったところClostridium属菌の新種であったことから、Clostridium folliculivoransと命名した。本細菌ゲノム中の構造遺伝子には、米ぬか成分であるスターチ等のバイオマスを分解する酵素遺伝子を複数個存在した。また本細菌は、嫌気条件を好む同菌属としては珍しく強い酸素耐性能を示した。2）単離細菌や既知の細菌などClostridium属細菌を米ぬかと培養し、培養液中の揮発性成分を香気捕集剤により抽出しGC-MSで網羅的に一斉解析した。その結果、コナギ種子に対して発芽阻害を示す培養液中からは酢酸と酪酸が阻害濃度で検出された。また、コナギ発芽率を目的変数、各成分の積分値を説明変数としたPLS揮発性成分分析の結果、変数重要度の高い成分として酢酸・酪酸を含む短鎖脂肪酸とエタノール、エチルエステル類が示された。また、多塩基酸ジエチルには阻害作用の緩和との関連が示唆された。3）水稲有機栽培圃場試験および有機水田土壌を使用したポット試験において、米ぬか散布後に経時的に土壌溶液および表層土壌を採取するとともに、コナギ防除効果を評価した。土壌溶液および土壌サンプルは化学分析およびDNA解析のために冷凍保存し、順次解析中である。

The main results are as follows: 1) under the condition of long-term organic water cultivation, the growth of paddy field soil was not suitable for long-term cultivation, and under the condition of disrelish, the method of plate culture was used to detect the activity of bud inhibition in paddy field. The new species of bacteria belonging to the genus Clostridium are identified in this paper, and the names of the new species of the genus Clostridium folliculivorans are identified. In this strain, there are several enzymes in the production of yeast, the components of rice, and the decomposition of enzymes in this bacteria. This fungus, disrelish that the condition is good, and the bacteria of the same genus are rare, and the acid tolerance is not strong. 2) it is known that the isolated bacteria belong to the genus Clostridium, and the sexual components in the culture solution are collected and extracted from the GC-MS network. The results showed that there was a significant difference in the degree of damage caused by oxalic acid and butyric acid in the solution. The results of PLS sexual composition analysis, the high degree of numerical importance, the content of oxalic acid and butyric acid, the content of short fatty acid, the content of fatty acid, the number of fatty acids, the results of sexual composition analysis, the results of chemical composition analysis, the results of sexual composition analysis, the results of chemical composition analysis, and the results of chemical composition analysis. Polybasic acids and polybasic acids show signs of inhibition and instigation. 3) the organic cultivation nursery and nursery yard of water and the soil of the paddy field can be used for the prevention and control of fruit pollution by using the solution of the soil after the spread of rice and rice. Soil solution, chemical analysis, DNA analysis, cold preservation, secondary analysis.

项目成果

抑草剤

草药抑制剂

• 发表时间: 2022

Clostridium folliculivorans sp. nov., isolated from soil samples of an organic paddy in Japan.

• DOI: 10.1099/ijsem.0.005876
• 发表时间: 2023-04
• 期刊: International journal of systematic and evolutionary microbiology
• 影响因子: 2.8
• 作者: J. Tazawa;Hisami Kobayashi;Yasuhiro Tanizawa;A. Uchino;F. Tanaka;Y. Urashima;S. Miura;M. Sakamoto-M.-Sakamot