Revealing the Role of Postnatal Theta Rhythm in Establishing Memory Consolidation Mechanism during REM Sleep

揭示产后 Theta 节律在快速眼动睡眠期间建立记忆巩固机制中的作用

• 批准号: 21K06419
• 负责人: Kumar Deependra
• 金额: $ 2.58万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21K06419/
• 关键词: Sleep; memory; Theta; hippocampus; neonates; TRAP2; Dreamless; Fear expression; Neonatal sleep; Neonatal memory; Theta rhythm maturation; Dreamless mice; Engram

We found dreamless mice display low freezing behavior during fear conditioning, which could be due to chronic loss of REM sleep. Early data indicates their REM sleep duration gets further shortened during the postconditioning sleep, which can influence the memory consolidation. Furthermore, hippocampal theta shows low spectral power during REM sleep, that required for memory storage.Indeed dreamless show low-freezing behavior one day or two weeks later. Dreamless shows a larger loss of memory at two weeks time, indicating faster memory decay compared with wild type littermates . Upon remote memory retrieval, the number of c-fos positive cells in the anterior cingulate cortex (ACC), a center for remote memory storage, was low in dreamless, which agrees with lower freezing behavior.

我们发现，无梦小鼠在恐惧条件反射过程中表现出低冰冻行为，这可能是由于长期丧失快速眼动睡眠。早期数据表明，在后条件睡眠过程中，他们的REM睡眠持续时间进一步缩短，这可能会影响记忆的巩固。此外，在REM睡眠期间，海马theta显示出低光谱能量，这是存储记忆所需的。真正的无梦在一天或两周后表现出低冰冻行为。在两周的时间里，无梦猫表现出更大的记忆力丧失，这表明与野生型猫咪相比，记忆衰退更快。在远距离记忆提取时，无梦状态下扣带回前部(ACC)c-fos阳性细胞数量较少，这与较低的冰冻行为相一致。

项目成果

Kinase signalling in excitatory neurons regulates sleep quantity and depth

• DOI: 10.1038/s41586-022-05450-1
• 发表时间: 2022-12-07
• 期刊: NATURE
• 影响因子: 64.8
• 作者: Kim，Staci J.;Hotta-Hirashima，Noriko;Funato，Hiromasa
• 通讯作者: Funato，Hiromasa