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Structural and functional analysis of mating type locus encoded transcription factors from the Penicillia

青霉菌交配型位点编码转录因子的结构和功能分析

基本信息

批准号：
299103792
负责人：
Professor Dr. Ulrich Kück
金额：
--
依托单位：
Lehrstuhl für Allgemeine und Molekulare Botanik
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2016
资助国家：
德国
起止时间：
2015-12-31 至 2020-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/299103792?language=en
关键词：
Structural functional analysis mating type

项目摘要

Although the genus Penicillium provides us with some of the most important fungal species used in applied microbiology, only little is known about their developmental peculiarities in terms of sexual reproduction. Moreover, the observation that regulatory functions of mating type locus (MAT) encoded transcription factors (TFs) reach far beyond the orchestration of processes directly related to mating emphasizes their relevance for biotechnology, and therefore the need for further analysis of MAT controlled cellular and developmental functions. Besides the functional and structural characterization of MAT TFs, a comprehensive knowledge of gene regulatory networks controlled by MAT proteins as well as the ability to induce the sexual life cycle in various Penicillium species, are of major importance. We want to get insight into the cooperative interaction of MAT TFs with their specific target DNA and other proteins. We will therefore address the following questions: a.) What are the interaction partners of MAT TFs on the protein level? and b.) What is the X-ray structure of the alpha-domain MAT1-1-1 TF from P. chrysogenum?.We are further interested in mating type systems and sexual life cycles of members of the Penicillia, other than P. chrysogenum. We will ask: a.) How do MAT TFs mediate the time and development-dependent expression of their specific target genes in different Penicillium species? and b.) Is there a sexual life cycle in two Penicillium species, used in applied microbiology, namely P. brevicompactum and P. citrinum?

尽管青霉菌属为我们提供了一些应用微生物学中最重要的真菌物种，但人们对它们在有性生殖方面的发育特点知之甚少。此外，对交配型位点（MAT）编码转录因子（tf）的调控功能的观察远远超出了与交配直接相关的过程的协调，强调了它们与生物技术的相关性，因此需要进一步分析MAT控制的细胞和发育功能。除了了解MAT tf的功能和结构特征外，全面了解由MAT蛋白控制的基因调控网络以及在各种青霉菌物种中诱导性生命周期的能力也非常重要。我们希望深入了解MAT tf与其特定靶DNA和其他蛋白质的合作相互作用。因此，我们将解决以下问题：a.)MAT tf在蛋白质水平上的相互作用伙伴是什么？和b)。P. chrysogenum α -域MAT1-1-1 TF的x射线结构是什么？我们进一步感兴趣的交配类型系统和青霉菌成员的性生命周期，除了P. chrysogenum。我们会问：a.)MAT tf如何介导不同青霉菌中特定靶基因的时间依赖性和发育依赖性表达？和b)。在应用微生物学中使用的两种青霉菌，即P. breviccompacactum和P. citriinum，是否存在有性生命周期？

项目成果

期刊论文数量（11）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Mating-Type-Specific Ribosomal Proteins Control Aspects of Sexual Reproduction in Cryptococcus neoformans

交配型特异性核糖体蛋白控制新生隐球菌有性生殖的各个方面

DOI：
10.1534/genetics.119.302740
发表时间：
2020
期刊：
Genetics
影响因子：
3.3
作者：
Ianiri G;Fang YF;Dahlmann TA;Clancey SA;Janbon G;Kück U;Heitman J
通讯作者：
Heitman J

Rampant transposition following RNAi loss causes hypermutation and antifungal drug resistance in clinical isolates of a human fungal pathogen

DOI：
10.1101/2021.08.11.455996
发表时间：
2021-08
期刊：
bioRxiv
影响因子：
0
作者：
Shelby J. Priest;V. Yadav;Cullen Roth;Tim A. Dahlmann;U. Kück;Paul M. Magwene;J. Heitman
通讯作者：
Shelby J. Priest;V. Yadav;Cullen Roth;Tim A. Dahlmann;U. Kück;Paul M. Magwene;J. Heitman

Construction of a Codon-Adapted Nourseotricin-Resistance Marker Gene for Efficient Targeted Gene Deletion in the Mycophenolic Acid Producer Penicillium brevicompactum