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Coordination and interaction of the STRIPAK complex during sexual differentiation

STRIPAK复合物在性分化过程中的协调和相互作用

基本信息

批准号：
157339925
负责人：
Professor Dr. Ulrich Kück
金额：
--
依托单位：
Lehrstuhl für Allgemeine und Molekulare Botanik
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2009
资助国家：
德国
起止时间：
2008-12-31 至 2016-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/157339925?language=en
关键词：
Coordination interaction STRIPAK complex during

项目摘要

Living organisms are complex systems that have the constant capacity to response to alternating signals. As a consequence, organisms will undergo major morphological and physiological changes that can be recognized for example at the molecular level by the dynamics of protein complexes. This project focusses on components of high molecular weight complexes that are shared between different signaling pathways and thus talk to each other through so far unknown mechanisms. A key finding of the previous funding period is the discovery of the STRIPAK protein complex, controlling sexual development in the model fungus Sordaria macrospora. This ascomycete provides the opportunity to study distinct subunits of the STRIPAK complex, using molecular, genetic, biochemical and/or cell biological approaches. One subunit of STRIPAK is PRO45, a membrane associated subunit, showing significant homology to the human Sarcolemmal membrane-associated protein (SLMAP), which is believed to serve as physical and signaling connection between the Golgi and centrosomes and is important for spindle assembly. Other components of the STRIPAK complex are three subunits of protein phosphatase PP2A, and we will investigate the modulatory function of catalytic subunit PP2Ac1. Besides that we are interested, how the STRIPAK complex interacts with other complexes, involved in cell signaling. Selected Proteins are EPS12 and NDK1, which most probably cross-talk with other signaling cascades and thus allow the adaptation in response to varying signals. The STRIPAK complex is highly conserved within eukaryotes and therefore results obtained with the fungal experimental system will be of general relevance for other eukaryotic systems.

生物体是复杂的系统，具有对交替信号做出持续反应的能力。因此，生物体将经历重大的形态和生理变化，这些变化可以通过蛋白质复合物的动力学在分子水平上进行识别。该项目重点关注不同信号通路之间共享的高分子量复合物的成分，从而通过迄今为止未知的机制相互交流。上一资助期的一个重要发现是发现了 STRIPAK 蛋白复合物，该复合物控制模型真菌 Sordaria macrospora 的性发育。该子囊菌提供了使用分子、遗传、生化和/或细胞生物学方法研究 STRIPAK 复合物的不同亚基的机会。 STRIPAK 的一个亚基是 PRO45，这是一种膜相关亚基，与人类肌膜相关蛋白 (SLMAP) 显示出显着的同源性，SLMAP 被认为是高尔基体和中心体之间的物理和信号连接，对于纺锤体组装很重要。 STRIPAK 复合物的其他成分是蛋白磷酸酶 PP2A 的三个亚基，我们将研究催化亚基 PP2Ac1 的调节功能。除此之外，我们感兴趣的是 STRIPAK 复合物如何与参与细胞信号传导的其他复合物相互作用。选定的蛋白质是 EPS12 和 NDK1，它们很可能与其他信号级联串扰，从而允许适应不同的信号。 STRIPAK 复合物在真核生物中高度保守，因此真菌实验系统获得的结果与其他真核系统具有普遍相关性。