Molecular mapping of lipid biomarkers with Time-of-Flight - Secondary Ion Mass Spectrometry (TOF-SIMS): a feasibility study.

使用飞行时间-二次离子质谱 (TOF-SIMS) 进行脂质生物标志物的分子图谱：可行性研究。

• 批准号: 31230282
• 负责人: Professor Dr. Volker Thiel
• 金额: --
• 依托单位: Abteilung Geobiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2006-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/31230282?language=en
• 关键词: Molecular; mapping; lipid; biomarkers; Time

Time of Flight - Secondary Ion Mass Spectrometry (TOF-SIMS) has become a routine analytical tool in material research where it is mainly used for the characterisation of surface properties and composition. Specifically with the development of new polyatomic primary ion sources, TOF-SIMS can be used to perform high-quality chemical imaging by rastering over the sample surface and quasicontinuously collecting mass spectra. A molecular map of a sample area can thus be generated for any compound that produces a diagnostic mass peak in the spectrum. Although these advantageous features exactly meet the demands of many modern geobiological approaches, the potential of TOF-SIMS for such applications remains yet to be explored. In this project, we will systematically evaluate the capabilities and limits of TOF-SIMS in order to open a new analytical window in organic biomarker studies. Emphasis is placed on (1) the generation and interpretation of basic spectral reference data for major biolipids and geolipids, and (2) the identification and molecular mapping of these biomarkers in geobiological materials ranging from microbial mats to solid rocks. This project is designed as a feasibility study that will form the basis for a geobiological follow-on project on the sources and distributions of organic molecules in active microbial systems, and in related minerals and rocks.

飞行时间-二次离子质谱（TOF-SIMS）已成为材料研究中的常规分析工具，主要用于表征表面性质和成分。特别是随着新的多原子一次离子源的发展，TOF-SIMS可以通过在样品表面上光栅扫描和准连续收集质谱来进行高质量的化学成像。因此，对于在光谱中产生诊断质量峰的任何化合物，可以生成样品区域的分子图。虽然这些有利的功能完全满足了许多现代地球生物学方法的需求，TOF-SIMS在这些应用中的潜力仍有待探索。在这个项目中，我们将系统地评估TOF-SIMS的能力和局限性，以便在有机生物标志物研究中打开一个新的分析窗口。重点放在（1）主要生物脂和地质脂的基本光谱参考数据的生成和解释，以及（2）从微生物席到固体岩石的地质生物材料中这些生物标志物的识别和分子制图。该项目旨在作为一项可行性研究，为关于活跃微生物系统以及相关矿物和岩石中有机分子的来源和分布的地球生物学后续项目奠定基础。