Time-of-Flight - Secondary Ion Mass Spectrometry (ToF-SIMS): exploring the sources and distributions of microbial biomarkers at the µm-range

飞行时间-二次离子质谱 (ToF-SIMS)：探索 µm 范围内微生物生物标志物的来源和分布

• 批准号: 88430332
• 负责人: Professor Dr. Volker Thiel
• 金额: --
• 依托单位: Abteilung Geobiologie
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/88430332?language=en
• 关键词: Time; Flight; Secondary; Ion; Mass

Time of Flight - Secondary Ion Mass Spectrometry (ToF-SIMS) is a technique that detects inorganic and organic molecules on surfaces, without the need to extract the sample in question. Moreover, ToF-SIMS is able to display at the microscopic level the intensities of any ion detected, thus exceeding by far the resolution achievable with the extract-based techniques routinely used in biomarker studies. The objective of the current project is to provide basic research and experimental work to further advance the utility of ToF-SIMS imaging mass spectrometry for geobiological applications. During the first project term, fundamental data have been obtained on standard biomarker compounds and artificial experimental setups. In this renewal proposal, emphasis will be placed on using imaging mass spectrometry on environmental conditioning films, biofilms, and microbial mats sampled from the Äspö Hard Rock Laboratory and saline lakes on Kiritimati. In order to enhance the significance of the spectral data obtained, it is planned to link ToF-SIMS with statistical analysis and microscopic techniques, particularly conventional microscopy and scanning electron microscopy (SEM). Enhanced knowledge about the spatial localization of organic molecules at the microscopic level will enable a better understanding of the mechanisms of biomineralization, the complex ecological interactions in microbial systems, and will allow for a more clear-cut association of lipid biomarkers with their biological source.

飞行时间 - 次级离子质谱（TOF -SIMS）是一种检测表面上无机和有机分子的技术，而无需提取相关样品。此外，TOF-SIMS能够在微观级别显示任何检测到的离子的强度，因此超过了迄今为止使用用于生物标志物研究的基于提取物技术的分辨率。当前项目的目的是提供基础研究和实验性工作，以进一步推动TOF-SIMS成像质谱法对地球生物学应用的实用性。在第一个项目术语中，已经获得了标准生物标志物化合物和人工实验设置的基本数据。在此续签建议中，将重点放在对环境条件膜，生物膜和从基里蒂玛蒂（Kiritimati）上的äspö硬岩实验室和盐水湖泊采样的微生物垫上使用成像质谱法。为了增强获得的光谱数据的重要性，计划将TOF-SIM与统计分析和显微镜技术（尤其是常规显微镜和扫描电子显微镜（SEM））联系起来。关于微生物系统中有机分子的空间定位的增强知识将使对生物矿化的机制，微生物系统中的复杂生态相互作用，并使脂质生物标志物与其生物源建立更清晰的关联。