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持久的運動効果の減弱化のメカニズム解明とその解決策

阐明持续运动效果衰减机制及其解决方案

基本信息

批准号：
20K11417
负责人：
星野太佑
金额：
$ 2.75万
依托单位：
The University of Electro-Communications
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2020
资助国家：
日本
起止时间：
2020-04-01 至 2024-03-31
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20K11417/
关键词：
冷却骨格筋筋収縮 AMPK PGC-1a ミトコンドリア PGC-1α

项目摘要

これまで，遺伝子発現に着目して，冷却による持久的運動の効果の減弱化のメカニズムを検証してきた．今年度は，収縮中の冷却がタンパク質合成に与える影響に着目し検討した．Wistar系雄性ラットを常温群と冷却群にわけ，常温群は水, 冷却群は氷水の入った袋を用いて，右脚の下腿前部を3分間冷却した. その後, 前脛骨筋を20 Hzにて1秒間電気刺激, 1秒間休息を30回繰り返すプロトコルを1セットとし, これを10セット実施した. 筋収縮2.5時間後にpuromycinを腹腔投与し, 3時間後に両脚の前脛骨筋を摘出した．摘出した筋から，whole proteinと抽出したミトコンドリアのサンプルに対して，puromycin抗体を用いたウエスタンブロッティングを実施することで，タンパク質合成速度を定量化した．その結果，whole proteinを用いて定量化した場合，非収縮脚に対する収縮脚のタンパク質合成速度の増加率に，冷却による有意な違いはみられなかった．次に，抽出したミトコンドリアサンプルを用いて，ミトコンドリアのタンパク質合成速度を定量化した．抽出したミトコンドリアサンプルの妥当性については，細胞質やミトコンドリアのタンパク質をwhole proteinのサンプルと比較することで確認した．定量化した結果，非収縮脚に対する収縮脚のミトコンドリアタンパク質合成速度の増加率が，冷却により低下する傾向がみられた (p=0.055)．これらの結果から，冷却は筋収縮後のタンパク質全体の合成速度には影響を及ぼさないが，ミトコンドリアのタンパク質合成速度を低下させる可能性が示唆された．

The effect of the cooling system on the temperature of the cooling system is obvious, and the long-lasting effect of the cooling system weakens the temperature response of the cooling system. This year, the temperature of the cooling system in the middle of the year is high. The Wistar series of male cryostat is used in the room temperature group cooling group cooling system, the normal temperature group cooling water is used, and the cooling group cooling water is put into the tank bag. Cool the front of the lower leg for 3 minutes on the right foot. After that, the front tendon was stimulated for 20 Hz per second, 30 times in 1 second for rest, and 1 minute for 10 minutes. After 2.5 hours, the puromycin was injected intraperitoneally, and after 3 hours, the tendons were extracted from the front of the feet. The tendons were removed, and the whole protein was extracted. The tendons were extracted, and the puromycin antibodies were used to quantify the synthesis rate. The results showed that the whole protein was closed with a quantitative assay. The speed of synthesis is not affected by the number of feet. The speed of synthesis is not affected by the increase rate of synthesis speed. In the second time, the synthesis speed is quantified, and the synthesis speed is quantified. The second time, pull out the appropriate speed. The results of the quantitative analysis of the results of the quantitative results showed that the synthesis speed was significantly higher than that of the control group, the synthesis rate was significantly higher than that of the control group, the synthesis rate was significantly higher than that of the control group, and the synthesis rate was significantly higher than that of the control group (p < 0.05). The results of the quantitative results showed that the synthesis speed was significantly higher than that of the control group (p < 0.05). After cooling the ribs, we need to know the synthesis speed, the speed of synthesis, the speed of synthesis and the speed of synthesis.