Analysis of TCP transcription factors and downstream regulatory networks in the basal land plant Marchantia polymorpha

陆生植物地钱TCP转录因子及下游调控网络分析

• 批准号: 313526816
• 负责人: Professorin Dr. Sabine Zachgo
• 金额: --
• 依托单位: Arbeitsgruppe Botanik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/313526816?language=en
• 关键词: Analysis; TCP; transcription; factors; downstream

Arabidopsis thaliana TCP genes are transcription factors that regulate cell division processes and shape thereby the plant architecture. Recently, further functions in signaling processes and plant immunity have been reported. However, knowledge about the evolution of their divers activities is lacking. The basal land plant Marchantia polymorpha is a liverwort with a crucial, informative phylogenetic position. Marchantia has been established as a novel genetic model organism and comprises almost all gene families, however with low gene numbers. 21 TCP genes exist in Arabidopsis and only two in Marchantia, avoiding gene redundancy effects known to hamper other functional TCP analyses. The goal of this project is to unravel the TCP functions by analyzing knockout and knockdown mutants. Downstream regulatory networks will be identified and selected target genes will be further characterized. Phylogeny reconstructions will shed light on the evolution of TCP genes and enable to identify interesting motives and amino acids. These analyses aim to conduct comparative studies with eudicot TCP genes to understand the molecular evolution of their functions and how they contributed to the formation of increasingly complex land plants.

拟南芥的tcp基因是一种转录因子，调节细胞分裂过程，从而塑造植物的结构。近年来，在信号转导过程和植物免疫方面的进一步功能已有报道。然而，对他们潜水员活动的演变缺乏了解。地钱是一种地钱植物，具有重要的系统发育地位。Marchantia是一种新型的遗传模式生物，包含了几乎所有的基因家族，但基因数量较少。拟南芥中存在21个TCP基因，Marchantia中只有2个，从而避免了已知的阻碍其他功能TCP分析的基因冗余效应。这个项目的目标是通过分析基因敲除和基因敲除突变来解开tcp的功能。将确定下游调控网络，并对选定的目标基因进行进一步表征。系统发育重建将阐明TCP基因的进化，并能够识别有趣的动机和氨基酸。这些分析旨在与eudicot tcp基因进行比较研究，以了解它们功能的分子进化，以及它们如何促成日益复杂的陆地植物的形成。

项目成果

Insights into Land Plant Evolution Garnered from the Marchantia polymorpha Genome

• DOI: 10.1016/j.cell.2017.09.030
• 发表时间: 2017-10-05
• 期刊: CELL
• 影响因子: 64.5
• 作者: Bowman, John L.;Kohchi, Takayuki;Schmutz, Jeremy
• 通讯作者: Schmutz, Jeremy

The Chara Genome: Secondary Complexity and Implications for Plant Terrestrialization

• DOI: 10.1016/j.cell.2018.06.033
• 发表时间: 2018-07-12
• 期刊: CELL
• 影响因子: 64.5
• 作者: Nishiyama, Tomoaki;Sakayama, Hidetoshi;Rensing, Stefan A.
• 通讯作者: Rensing, Stefan A.