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Analysis of a novel plant glutaredoxin involved in flower development

一种参与花卉发育的新型植物谷氧还蛋白的分析

基本信息

批准号：
12529691
负责人：
Professorin Dr. Sabine Zachgo
金额：
--
依托单位：
Arbeitsgruppe Botanik
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2005
资助国家：
德国
起止时间：
2004-12-31 至 2015-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/12529691?language=en
关键词：
Analysis novel plant glutaredoxin involved

项目摘要

Although much research has been conducted on the function of homeotic class A, B and C genes controlling flower organogenesis, little is still known about the genes required for the initiation of the flower organ primordia, an event which is not controlled by these key regulatory genes. We have isolated a T-DNA mutant producing a reduced number of petal primordia and showing abnormalities during later petal development, such as bending of the petal blade. Surprisingly, the affected gene, named ROXY1, encodes a glutaredoxin (GRX) and belongs to a large Arabidopsis gene family for which information is scarce. GRXs are oxidoreductases that oxidize or reduce conserved, cysteine containing motives. Thus far, GXRs have been associated with the response to oxidative stress. Our data indicate for the first time that a GRX plays a crucial role during flower development, likely by means of a conserved cysteine mediating posttranslational modifications. We want to take advantage of the first characterization of a GRX mutant and investigate the function of ROXY1 and related GRXs in detail. The spectrum of ROXY1 target genes seems to be rather broad, as indicated by the roxy1 mutant phenotype and by genetic interactions studies. We consider that it is of particular interest to identify and characterize target proteins to unravel the intriguing connection between redox regulation and flower development.

虽然对同源异体A、B和C类基因控制花器官发生的功能进行了大量研究，但对花器官原基起始所需的基因知之甚少，这一事件不受这些关键调控基因的控制。我们已经分离出一种T-DNA突变体，其产生的花瓣原基数量减少，并且在花瓣后期发育过程中表现出异常，例如花瓣叶片弯曲。令人惊讶的是，受影响的基因被命名为ROXY1，它编码谷氨酰胺还蛋白（GRX），属于一个大的拟南芥基因家族，关于这个基因家族的信息很少。grx是氧化还原酶，可以氧化或还原含有半胱氨酸的保守动机。到目前为止，gxr与氧化应激反应有关。我们的数据首次表明GRX在花的发育过程中起着至关重要的作用，可能是通过保守的半胱氨酸介导翻译后修饰。我们想利用GRX突变体的第一个特征，详细研究ROXY1和相关GRX的功能。从ROXY1突变体表型和遗传相互作用的研究来看，ROXY1靶基因的谱似乎相当广泛。我们认为鉴定和表征靶蛋白以揭示氧化还原调控与花发育之间的有趣联系是特别有趣的。