Development of a human cell-based in-vitro granuloma model to dissect the mechanism of TB reactivation

开发基于人体细胞的体外肉芽肿模型来剖析结核病再激活的机制

• 批准号: 20K16258
• 负责人: 郭 姿君
• 金额: $ 2.33万
• 依托单位: 公益財団法人結核予防会 結核研究所
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2020
• 资助国家: 日本
• 起止时间: 2020-04-01 至 2022-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20K16258/
• 关键词: LTBI; TB reactivation; In-vitro granuloma; TB Granuloma

To establish the human cell-based in-vitro granuloma model, human donated PBMCs were purchased from the company (Cosmo Bio) and stored in the liquid nitrogen until use. Other necessary reagents for the optimal growth of PBMCs to form granulomas, such as 3D culture gel, human serum for culture medium, and extracellular matrices, enable to mimic in-vivo environments for 3D cell culture applications were all obtained. Additionally, to develop a co-culture system for the dynamic interaction of the formulated granuloma and other immune cells in PBMCs, permeable Transwell Inserts were ordered. Prolonged survivals of granulomas will be used as an indicator for evaluating the feasibility of this co-culture system.The research was discontinued when the principal investigator was unable to continue the research beyond one year.

为了建立基于人细胞的体外肉芽肿模型，从公司（Cosmo Bio）购买人捐献的PBMC并储存在液氮中直至使用。获得了用于PBMC最佳生长以形成肉芽肿的其他必要试剂，例如3D培养凝胶、用于培养基的人血清和细胞外基质，其能够模拟用于3D细胞培养应用的体内环境。此外，为了开发一种用于配制的肉芽肿和PBMC中的其他免疫细胞的动态相互作用的共培养系统，订购了可渗透的Transwell培养器。肉芽肿的长期存活将被用作评估该共培养系统可行性的指标。当主要研究者无法继续研究超过一年时，该研究被终止。