Central project - Cross-project research, production and characterization of new model proteins

中心项目——新模型蛋白的跨项目研究、生产和表征

• 批准号: 315460011
• 负责人: Professor Dr. Dieter Jahn
• 金额: --
• 依托单位: Institut für Partikeltechnik
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/315460011?language=en
• 关键词: Central; project; Cross; research; production

The main goals of the Central Project are the support of SPP projects on the one hand by measuring techniques for the characterization of mechanical properties of proteins, on the other hand to develop production and purification protocols for different ß-lactoglobuline bovine (BlgB) variants via systematic scouting investigations in the μL-scale. After scaling up of the production and purification procedure BlgB-variants are distributed to other SPP projects for comparative studies. Thus, the Central Project promotes interdisciplinary scientific exchange in the SPP consortium.The most frequently used model proteins in the SPP represent BlgB or its defined variants. Accordingly, these proteins will be produced and characterized in the second phase of the SPP in order to make them available for the working groups in the required form (in dissolved or crystal form). In this way, proteins that differ in a defined manner are available for comparative studies and enable a comprehensive scientific exchange.Therefore, the techniques and materials used are focused on the determination of mechanical properties of proteins with AFM and nanoindenter as well as the preparation and purification of different BlgB variants. Also alcohol dehydrogenase (ADH) and other techniques will be used in the Central Project.

该中心项目的主要目标是一方面通过表征蛋白质机械特性的测量技术支持SPP项目，另一方面通过μ L规模的系统性探索研究开发不同牛β-乳球蛋白（BlgB）变体的生产和纯化方案。在扩大生产和纯化程序后，将BlgB变体分配给其他SPP项目进行比较研究。因此，中心项目促进SPP联盟中的跨学科科学交流。SPP中最常用的模型蛋白代表BlgB或其定义的变体。因此，这些蛋白质将在SPP的第二阶段进行生产和表征，以便以所需的形式（溶解或晶体形式）提供给工作组。通过这种方式，不同的蛋白质可以进行比较研究，并进行全面的科学交流。因此，所使用的技术和材料主要集中在使用AFM和纳米压痕仪测定蛋白质的机械性能，以及制备和纯化不同的BlgB变体。此外，乙醇脱氢酶（ADH）和其他技术将用于中央项目。