Molecular analysis and universality exploration of DNA damage-triggered reprogramming to stem cells
DNA损伤触发干细胞重编程的分子分析和普遍性探索
基本信息
- 批准号:21F21084
- 负责人:
- 金额:$ 1.47万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for JSPS Fellows
- 财政年份:2021
- 资助国家:日本
- 起止时间:2021-04-28 至 2023-03-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Our study aimed to understand the molecular mechanism of DNA damage-triggered reprogramming from differentiated leaf cells into stem cells in Physcomitrium patens. To this end, we conducted single cell transcriptome analysis through single nucleus RNA-sequencing (snRNA-seq) to track transcriptional changes during the reprogramming process. We successfully obtained high quality snRNA-seq data. After the clustering of nuclear transcriptomes, we obtained a set of genes which were specifically expressed in each cluster. With fluorescence observation of promoter reporter lines of these specifically expressed genes, multiple clusters were annotated to specific tissues. Based on these cluster annotation results, we can extract the reprogramming trajectory of nuclei from leaf cells to newly formed protonema stem cells. From this analysis, we can identify stage-specific genes during DNA damage-triggered reprogramming and differentially expressed genes between reprogramming and non-reprogramming leaf cells at the late stage, which will provide a potential list of key genes driving the cellular reprogramming.To explore the universality of DNA damage-triggered reprogramming in land plants, we established culture conditions for multiple angiosperms and treated each plant with Zeocin. In most cases, the DNA damage reagent caused cell death in the tested plants, suggesting that efficient DNA damage repair is critical for successful cellular reprogramming.
本研究旨在了解立碗藓分化叶细胞向干细胞DNA损伤触发重编程的分子机制。为此,我们通过单核RNA测序(snRNA-seq)进行单细胞转录组分析,以跟踪重编程过程中的转录变化。我们成功地获得了高质量的snRNA-seq数据。在核转录组聚类后,我们获得了一组在每个聚类中特异表达的基因。通过对这些特异表达基因的启动子报告基因系的荧光观察,将多个簇注释到特定组织。基于这些聚类注释结果,我们可以提取从叶细胞到新形成的原丝体干细胞的细胞核重编程轨迹。通过分析,我们可以确定DNA损伤触发重编程过程中的阶段特异性基因,以及重编程和非重编程叶细胞在后期的差异表达基因,这将提供一个潜在的驱动细胞重编程的关键基因列表。为了探索DNA损伤触发重编程在陆地植物中的普遍性,我们建立了多种被子植物的培养条件,并用Zeocin处理每种植物。在大多数情况下,DNA损伤试剂导致测试植物中的细胞死亡,这表明有效的DNA损伤修复对于成功的细胞重编程至关重要。
项目成果
期刊论文数量(13)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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玉田 洋介其他文献
植物の幹細胞化・再生メカニズムを解明する補償光学顕微鏡
自适应光学显微镜阐明植物干细胞化和再生的机制
- DOI:
- 发表时间:
2015 - 期刊:
- 影响因子:0
- 作者:
玉田洋介;服部雅之;早野裕;村田隆;大屋真;野中茂紀;亀井保博;長谷部光泰;玉田 洋介;玉田 洋介 - 通讯作者:
玉田 洋介
Relativistic study on the linearity of uranyl molecule
铀酰分子线性的相对论研究
- DOI:
- 发表时间:
2020 - 期刊:
- 影响因子:0
- 作者:
吉田 優佳;友井 拓実;大江 駿;坂本 丞;亀井 保博;玉田 洋介;Ayaki Sunaga - 通讯作者:
Ayaki Sunaga
Lignification occurs after perceiving haustorium inducing factors to facilitate prehaustorium induction in Striga hermonthica
在独脚金感知吸器诱导因子以促进吸器前诱导后发生木质化
- DOI:
- 发表时间:
2022 - 期刊:
- 影响因子:0
- 作者:
玉田 洋介;Nan Gu;Yuichiro Takahashi;Songkui Cui,Satoko Yoshida - 通讯作者:
Songkui Cui,Satoko Yoshida
玉田 洋介的其他文献
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{{ truncateString('玉田 洋介', 18)}}的其他基金
Elucidation of mechanisms underlying mechanical stimulus perception and Ca2+ propagation by micromechanical stimulation in living cells
阐明活细胞中微机械刺激机械刺激感知和 Ca2+ 传播的机制
- 批准号:
23K18133 - 财政年份:2023
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Challenging Research (Exploratory)
Elucidation of the principle of cellular reprogramming in multicellular organisms
阐明多细胞生物中细胞重编程的原理
- 批准号:
21H02499 - 财政年份:2021
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
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