Integrated analysis of mineral ion dynamics in rice crop exposed to acute herbivory stress

急性食草胁迫下水稻作物矿物离子动态的综合分析

• 批准号: 21H02196
• 负责人: Ivan Galis
• 金额: $ 11.23万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21H02196/
• 关键词: mineral; herbivory; defense; Mineral; Herbivory; Defense

We have been focusing on the role of silicon (Si), nickel (Ni) and heavy metal cadmium (Cd), and amino acid transport, in response to insect feeding. (1) The role of Si was investigated using hydroponic culture system supplied with Si and without Si. Larvae of chewing herbivore Mythimna loreyi performed significantly better on Si-free rice that confirmed previous reports. We further determined the minimum time for Si supply that was sufficient to restore anti-herbivore resistance in rice. We also investigated the role of Si in release of volatile signals from rice and effect of Si on gene expression in rice. (2) We found that Ni uptake can be stimulated by herbivore feeding. Therefore, a possibly new role of Ni in defense was investigated. In particular, a possible connection between function of Ni as a cofactor of urease enzyme in nitrogen metabolism was proposed. This was further supported by finding that the expression of urea transporter in rice is stimulated by insect herbivory. (3) Additionally, we investigated the role of Cd in direct plant defense based on the observation that Cd uptake was transiently induced in rice after attack of chewing and sucking herbivores. (4) T0 generation of CRISPR/Cas9 knock-out line of a highly inducible amino acid transporter by herbivory was constructed. (5) The role of nitrogen containing metabolites in plant defense was carried out by Dr. Aboshi. (6) The role of Si transporters in rice has been further clarified by Dr. Mitani, in particular the role of SIET proteins.

我们一直在关注硅(Si)、镍(Ni)和重金属镉(Cd)在昆虫取食中的作用，以及氨基酸的运输。(1)在加硅和不加硅的水培培养体系中，研究了硅的作用。食草性食草动物粘虫幼虫在无硅大米上的表现明显好于之前的报道。我们进一步确定了恢复水稻对食草动物抗性的最短供硅时间。我们还研究了硅在水稻挥发性信号释放中的作用以及硅对水稻基因表达的影响。(2)我们发现草食动物的摄食可以刺激镍的吸收。因此，研究了镍在防御中可能的新作用。特别是提出了镍作为尿素酶在氮代谢中的辅因子作用之间的可能联系。这一发现进一步支持了水稻中尿素转运蛋白的表达受到昆虫食草性刺激的结果。(3)此外，我们还观察到在食草动物攻击后，水稻对Cd的吸收被瞬时诱导，从而研究了Cd在植物直接防御中的作用。(4)构建了草食性高度诱导氨基酸转运蛋白基因敲除系CRISPR/Cas9的T0代。(5)Aboshi博士研究了含氮代谢产物在植物防御中的作用。(6)三谷博士进一步阐明了硅转运蛋白在水稻中的作用，特别是SIET蛋白的作用。

项目成果

Systemic defense against herbivores in rice: Not really the case

水稻对食草动物的系统防御：事实并非如此

• 发表时间: 2022
• 作者: Ivan Galis;Taiga Kuwabara;Yuko Hojo;David Wari;Tomonori Shinya
• 通讯作者: Tomonori Shinya

Dynamics of Silicification and the Role of Silicon in Rice Defense against Chewing and Sucking Herbivores

硅化动力学和硅在水稻防御咀嚼和吸吮食草动物中的作用

• 发表时间: 2022
• 作者: Dandy Ahamefula Osibe;Yuko Hojo;Tomonori Shinya;Ivan Galis
• 通讯作者: Ivan Galis

Lessons from rice defense against herbivores: What really matters?

水稻防御食草动物的经验教训：真正重要的是什么？

• 发表时间: 2023
• 作者: Galis;I.
• 通讯作者: I.

Si in rice: a long journey from uptake to deposition

水稻中的硅：从吸收到沉积的漫长旅程

• 发表时间: 2023
• 作者: Mitani-Ueno;N.
• 通讯作者: N.

Integrated view of plant metabolic defense with particular focus on chewing herbivores

植物代谢防御的综合观点，特别关注咀嚼食草动物

• DOI: 10.1111/jipb.13204
• 发表时间: 2022
• 期刊: Journal of Integrative Plant Biology
• 影响因子: 11.4
• 作者: Wari David;Aboshi Takako;Shinya Tomonori;Galis Ivan
• 通讯作者: Galis Ivan