Comprehensive study on the effects of periodontal pathogen on the pathophysiology of atrial fibrillation

牙周病原菌对房颤病理生理影响的综合研究

• 批准号: 21H03112
• 负责人: 宮内 睦美
• 金额: $ 11.15万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21H03112/
• 关键词: 歯周病原細菌; 心房細動; 歯周病; ビッグデータ解析; 心筋線維化; 歯性感染病巣; 歯周炎; Porphyromonas gingivalis; 心線維化; ジンジパイン

研究1：P.g.歯性感染が心房線維化に及ぼす影響を検討するためにC57BL/6マウスを通常食【Cont(対照)、 P.g.、 P.g.-Inh(治療)】と高脂肪食投与【HFD、 HFD-P.g.、 HFD-P.g.-Inh群】に分け、P.g.-inh群には治療としてジンジパイン阻害剤を毎日経口投与した。既に、P.g.歯性感染6週後と12週後のサンプルを回収し、解析中である。研究4：2021度までに心房細動（AF）に対してカテーテルアブレーション治療を受け、同意の得られた295症例について、歯周炎の程度、心房細動の病態、治療成績、予後及び手術時血清を用いたP.g.血清抗体価を解析し、歯科治療後の経過を前向きに追跡調査中である。研究5:手術中に左心耳切除を受けた患者64例（AFなし（N＝22）、発作性AF（N＝16）、非発作性AF（N＝26）を登録し、切除した左心耳切片の線維化領域（%）をアザン染色で評価した。その結果、発作性および非発作性AF患者は、AFのない患者よりも左心耳の線維化％が高かった（p = .005およびp < .0001）。非発作性AF患者では、左心耳血栓を有する患者は有しない患者に比べ左心耳線維化％が有意に高く（p = .002）、左心耳組織の線維化がAFの発症に関連する事を組織学的に明らかにした。また、AF患者における歯周病原細菌P.g.と左心耳の心エコーによる血栓の有無との関係を明らかにすることを目的としAFのアブレーション手術候補者569 症例を登録した。P. gingivalis fimAサブタイプ（タイプI～IV）の血清抗体価を測定し、P.g.血清科抗体価高値群と低値群に分類した。多変量回帰分析の結果、適切な抗凝固療法を受けているAF患者においてP.g.タイプIIおよびIVは左心耳心エコーの重症度と関連していた。

Study 1:P.g. Patients with sexual infection usually eat high fat diet [Cont (photo), P.G., P.g.-Inh (treatment)] and [HFD, HFD-P.g., HFD-P.g.-Inh group], and P.g.-inh group therapy. The results show that the diet of Cont (photo), P.G., P.g.-Inh (therapy), high fat diet and [HFD, HFD-P.g., HFD-P.g.-Inh group] diet is very important in the treatment of sexual infection. Both P.G. After 6 years of sexual infection and 12 years later, the patients returned to the hospital after 6 years of sexual infection. Study 4RV 2021 degree atrial exercise (AF). The patients agreed to receive the treatment. 295 patients suffered from chronic heart disease, the degree of periarthritis, the state of atrial movement, the treatment of the disease, the use of serum after treatment and during surgery. The serum antibodies were analyzed, and the serum antibodies were traced forward after treatment. Study 5: patients who underwent surgical resection of left atrial appendage (AF 22), operational AF (16), non-invasive AF (26), resection of left atrial appendage (%). The results showed that the left atrial appendage (LAA) was significantly increased in patients with non-active AF and AF patients (p = .005). (P & lt;. 0001). Non-invasive AF patients, patients with left atrial appendage thrombosis, patients with left atrial appendage thrombus, patients with left atrial appendage thrombosis, patients with left atrial appendage thrombus, patients with left atrial appendage thrombosis, patients with left atrial appendage thrombus, patients with left atrial appendage thrombosis, patients with left atrial appendage thrombosis The pathogen P.G. Was isolated from the patients with chronic hepatitis B and AF. The left atrial appendage was complicated with thrombosis. There were no signs of thrombosis in the left atrial appendage. The purpose of this study was to detect AF symptoms in 569 patients with surgical symptoms. P. gingivalis fimA serum antibody test, P.G. (serum antibody I~IV). Serological antibodies were classified into high and low groups. The results of multi-dose regression analysis and the method of cutting anticoagulation were tested in patients with AF. II, IV, left atrial appendage, severity of the disease.

项目成果

Porphyromonas gingivalis and left atrial appendage spontaneous echo contrast in atrial fibrillation ablation candidates

房颤消融候选人中牙龈卟啉单胞菌和左心耳自发回声对比

• DOI: 10.1007/s00380-021-01851-w
• 发表时间: 2021
• 期刊: Heart and Vessels
• 影响因子: 1.5
• 作者: Miyauchi Shunsuke;Tokuyama Takehito;Shintani Tomoaki;Uotani Yukimi;Miyamoto Shogo;Ikeuchi Yoshihiro;Okamura Sho;Okubo Yousaku;Miyauchi Mutsumi;Nakano Yukiko
• 通讯作者: Nakano Yukiko

Periodontitis and the outcome of atrial fibrillation ablation: Porphyromonas gingivalis is related to atrial fibrillation recurrence

牙周炎与房颤消融的结果：牙龈卟啉单胞菌与房颤复发相关

• DOI: 10.1111/jce.14952
• 发表时间: 2021
• 期刊: Journal of Cardiovascular Electrophysiology
• 影响因子: 2.7
• 作者: Miyauchi Shunsuke;Tokuyama Takehito;Shintani Tomoaki;Nishi Hiromi;Hamamoto Yuta;Ouhara Kazuhisa;Furusho Hisako;Miyauchi Mutsumi;Komatsuzawa Hitoshi;Nakano Yukiko
• 通讯作者: Nakano Yukiko

Association between left atrial appendage fibrosis and thrombus formation: A histological approach

• DOI: 10.1111/jce.15384
• 发表时间: 2022-01-30
• 期刊: JOURNAL OF CARDIOVASCULAR ELECTROPHYSIOLOGY
• 影响因子: 2.7
• 作者: Miyauchi, Shunsuke;Tokuyama, Takehito;Nakano, Yukiko
• 通讯作者: Nakano, Yukiko