Genetic code expansion tools to study and control conformational dynamics and protein assembly (B10)

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By incorporating unnatural amino acids (UAA) bearing new functional moieties site-specifically into proteins it has become possible to leverage genetic code expansion approaches to address unmet challenges in studying and controlling biological processes. By combining the advantages of genetic code expansion with new chemoselective reactions, we aim to study and control biological systems under investigation within this CRC with a new level of spatial, temporal and molecular precision. We will develop UAA-based tools to investigate protein assembly and protein-protein interactions of small GTPases and the caseinolytic protease ClpP and elucidate folding and assembly of interleukins and small heat shock proteins.