Subsurface imaging of magnetic nanoparticles and quantification of nanomechanical properties of polymeric and biological materials by bimodal atomic force microscopy

通过双峰原子力显微镜对磁性纳米粒子进行地下成像并量化聚合物和生物材料的纳米机械性能

• 批准号: 318205773
• 负责人: Privatdozent Dr. Christian Dietz
• 金额: --
• 依托单位: Fachgebiet Physics of Surfaces
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/318205773?language=en
• 关键词: Subsurface; imaging; magnetic; nanoparticles; quantification

The main purpose of targeted drug delivery is to release the active substance directly at the target location. The exact diffusion mechanisms of the drug carrier, e.g. the motion processes of functionalized nanoparticles within the human body, however, are mainly unexplored. This demands a tool that enables the observation of diffusion processes in real-time and the correlation of the mechanical and biological properties of cellular tissue with the diffusion locations. For this purpose, the suggested project addresses the high-resolution characterization of mechanical properties of polymeric and biological materials, the diffusion processes of magnetic nanoparticles and their subsurface detection. Advanced atomic force microscopy (AFM) methods, such as frequency modulation combined with a simultaneous excitation of two or more cantilever eigenmodes are to be improved. To enhance the lateral resolution, the imaging rate and the sensitivity of the method, short cantilevers will be used, whose applicability should be demonstrated in the liquid environment. A photo thermal excitation of the cantilever prevents the stimulation of undesired resonances in the AFM setup. An improvement of the electronics of the system for faster imaging rates will allow the in situ observation of biological processes. To this end, a subsurface detection of magnetic nanoparticles in polymeric and biological materials will be realized. The combination of fast imaging rates with the quantification of the mechanical sample properties and structural and magnetic detection on the nanoscale, allows for the observation of diffusion processes of magnetic nanoparticles into cellular tissue. To visualize the penetration of nanoparticles through the cell membranes helps to gain new knowledge about the targeted drug delivery. The main goal of the project is to correlate the diffusion locations of the nanoparticles with the nanomechanical properties of endothelial cells.

靶向给药的主要目的是将活性物质直接释放到靶位置。然而，药物载体的确切扩散机制，例如功能化纳米颗粒在人体内的运动过程，主要是未探索的。这需要一种工具，能够实时观察扩散过程以及细胞组织的机械和生物特性与扩散位置的相关性。为此目的，建议的项目涉及聚合物和生物材料的机械性能的高分辨率表征，磁性纳米颗粒的扩散过程及其表面下的检测。先进的原子力显微镜（AFM）的方法，如频率调制与两个或两个以上的悬臂梁本征模的同时激发相结合，将得到改善。为了提高该方法的横向分辨率、成像速率和灵敏度，将使用短杠杆，其适用性应在液体环境中证明。的悬臂的光热激发防止在AFM设置中的不希望的共振的刺激。为了更快的成像速率而改进系统的电子设备将允许对生物过程进行原位观察。为此，将实现聚合物和生物材料中磁性纳米颗粒的亚表面检测。快速成像速率与纳米尺度上的机械样品性质和结构和磁性检测的量化相结合，允许观察磁性纳米颗粒扩散到细胞组织中的过程。可视化纳米颗粒穿过细胞膜的渗透有助于获得有关靶向药物递送的新知识。该项目的主要目标是将纳米颗粒的扩散位置与内皮细胞的纳米力学性质相关联。