Changes in cellular dynamics of cellulose synthases during secondary cell wall production

次生细胞壁生成过程中纤维素合酶的细胞动力学变化

• 批准号: 19K16168
• 负责人: 渡邊 洋一郎
• 金额: $ 2.66万
• 依托单位: Nara Institute of Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2019
• 资助国家: 日本
• 起止时间: 2019-04-01 至 2021-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-19K16168/
• 关键词: Cellulose; Plant Cell Wall; Plant Cell Biology; Molecular Biology; Microscopy; Secondary Cell Walls; Cell Biology

We have submitted a draft manuscript on one of our related research projects. This project has looked at the role of a protein closely associated with cellulose synthesis, but who’s function is still unknown, especially in plant secondary cell walls. Our paper presents data supporting the hypothesis that this protein is important for controlling the speeds at which enzymes produce cellulose. This may cause the defects in the crystallinity of the cellulose being produced when the protein is absent, resulting in the decrease in cellulose strength but not quantity. We are currently awaiting response from reviewers.We have created several photo-convertible fluorophore tagged cellulose synthase enzymes and tested their efficacy in plants. So far we have determined that though some of these fluorophores are viable in bacteria, yeast and mammalian systems, they are not viable in plants due to their weak signal and potential for dimerizing prematurely causing artifacts. We have however identified several that would work for our goals of visualizing the cellular dynamic turnover of cellulose synthase enzymes and are currently developing research questions with such tools including determining enzyme residency within Golgi bodies.Our experiments have showed negative data for the presence of lipid microdomains within the plasma membrane surrounding developing secondary cell walls. This data infers that there are no such domains and we are currently seeing if we can combine the data to publish in a short paper.

我们已经提交了一份关于我们的一个相关研究项目的草稿。该项目研究了与纤维素合成密切相关的蛋白质的作用，但其功能仍然未知，特别是在植物次生细胞壁中。我们的论文提供了支持这一假设的数据，即这种蛋白质对控制酶产生纤维素的速度很重要。当蛋白质不存在时，这可能导致所生产的纤维素的结晶度缺陷，导致纤维素强度降低，但数量不降低。我们目前正在等待评论者的回复。我们已经创造了几种光转换荧光标记的纤维素合成酶，并测试了它们在植物中的功效。到目前为止，我们已经确定，尽管这些荧光团中的一些在细菌、酵母和哺乳动物系统中是可行的，但它们在植物中是不可行的，这是由于它们的弱信号和过早二聚化导致伪影的可能性。然而，我们已经确定了几个，将为我们的目标可视化的细胞动态营业额的纤维素合成酶，目前正在开发的研究问题，这些工具，包括确定酶驻留在高尔基bodies. We的实验表明，负面数据的存在下，脂微区的质膜周围发育中的次生细胞壁。这些数据推断没有这样的域名，我们目前正在研究是否可以将这些数据联合收割机发表在一篇简短的论文中。

项目成果

Elucidating the molecular mechanism of COBRA-LIKE4 in secondary cell wall cellulose production

阐明 COBRA-LIKE4 在次生细胞壁纤维素生产中的分子机制

• 发表时间: 2019
• 作者: Yasui Y;Tsukamoto S;Sugaya T;Nishihama R;Wang Q;Kato H;Yamato KT;Fukaki H;Mimura T;Kubo H;Theres K;Kohchi T;Ishizaki K.;Yoichiro Watanabe
• 通讯作者: Yoichiro Watanabe

Identifying the cellular mechanism for CESA turnover during xylem differentiation

鉴定木质部分化过程中 CESA 周转的细胞机制

• 发表时间: 2019
• 作者: Ishio Hirono;Yoichiro Watanabe
• 通讯作者: Yoichiro Watanabe

Utilizing High-Resolution Live Cell Imaging to Elucidate the Cellular Mechanisms Controlling Plant Cellulose Deposition

利用高分辨率活细胞成像阐明控制植物纤维素沉积的细胞机制

• 发表时间: 2020
• 作者: Kouhei Oonuma and Takehiro G. Kusakabe;Yoichiro Watanabe
• 通讯作者: Yoichiro Watanabe

High-Resolution Live Cell Imaging of Developing Plant Protoxylem Reveals a Complex Mechanism Controlling Cellulose Deposition During Secondary Cell Wall Synthesis.

发育中植物 Protoxylem 的高分辨率活细胞成像揭示了次生细胞壁合成过程中控制纤维素沉积的复杂机制。

• 发表时间: 2020
• 作者: 大沼耕平;日下部岳広;Yoichiro Watanabe
• 通讯作者: Yoichiro Watanabe