Dynamics of complex membrane assemblies probed by native mass spectrometry (P24*)

通过天然质谱法探测复杂膜组件的动力学 (P24*)

• 批准号: 319163746
• 金额: --
• 依托单位: Institut für Physikalische und Theoretische Chemie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/319163746?language=en
• 关键词: Dynamics; complex; membrane; assemblies; probed

Crucial processes within cells depend on specific non-covalent interactions, which mediate the assembly of proteins and other biomolecules. We employ mass spectrometry and ion-mobility measurements to monitor protein stoichiometries, binding of ligands, lipid, and nucleotides as well as conformational changes and how these are interrelated. We will as well develop a method to investigate binding strength of membrane protein complexes in a quantitative way.

细胞内的关键过程取决于特定的非共价相互作用，介导蛋白质和其他生物分子的组装。我们采用质谱和离子迁移率测量来监测蛋白质的化学计量，配体，脂质和核苷酸的结合以及构象变化以及这些是如何相互关联的。我们也将发展一种方法，以定量的方式研究膜蛋白复合物的结合强度。