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Neural mechanisms underlying female sexual receptivity in medaka

青鳉雌性性接受的神经机制

基本信息

批准号：
20J13802
负责人：
Fleming Thomas
金额：
$ 1.09万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for JSPS Fellows
财政年份：
2020
资助国家：
日本
起止时间：
2020-04-24 至 2022-03-31
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20J13802/
关键词：
estrogen medaka pge2 receptor secretogranin 2 neuropeptide b female receptivity preoptic area FeSP neuron Medaka Neuroscience Prostaglandin E2 Estrogen Secretoneurin Carboxypeptidase 1

项目摘要

I have completed two projects studying the functions of estrogen-dependent genes (ptger4b and scg2a) expressed in medaka female-specific sex steroid-responsive peptidergic (FeSP) neurons. ptger4b-deficient females show increased receptivity to male courtship. I have determined that the FeSP neurons of ptger4b-deficient females show reduced spontaneous firing activity and accumulation of neuropeptides. We thus purposed a model where the estrogen-dependent expression of ptger4b in FeSP neurons controls their spontaneous firing activity which affects the release of neuropeptides important to female sexual receptivity.I have determined that the expression of scg2a in FeSP neurons lies downstream of the estrogen receptor subtype, Esr2b. Scg2a is thought to have dual roles as a factor controlling secretory granule biogenesis and as a precursor to pleiotropic signalling molecules. I analyzed the relationship between Scg2a and Npba (a neuropeptide expressed in FeSP neurons) and found that they co-localize in secretory granules. Further analysis showed that FeSP neurons of scg2a-deficient females do not show accumulation of Npba, changes in Npba subcellular localization, or changes in the total number of Npba-containing vesicles. This indicates that the role of Scg2a in FeSP neurons is most likely as a precursor to pleiotropic signalling molecules. I also analyzed the mating behaviour of scg2a-deficient medaka and found no differences between knockout and wild-type individuals, suggesting that Scg2a may be dispensable for or have no role in regulating mating behaviour.

我完成了两个项目，研究雌激素依赖基因(ptger4b和scg2a)在青冈雌性特异性类固醇反应肽(FeSP)神经元中表达的功能。Ptger4b基因缺失的雌性表现出对雄性求爱的接受度增加。我已经确定，ptger4b缺陷女性的FeSP神经元表现出自发放电活动减少和神经肽积累。因此，我们建立了一个模型，其中雌激素依赖的ptger4b在FeSP神经元中的表达控制其自发放电活动，从而影响对女性性接受至关重要的神经肽的释放。我已经确定在FeSP神经元中scg2a的表达位于雌激素受体亚型Esr2b的下游。Scg2a被认为具有控制分泌颗粒生物发生的因子和多效性信号分子的前体的双重作用。我分析了Scg2a和NPBA(一种在FeSP神经元中表达的神经肽)的关系，发现它们共同定位于分泌颗粒中。进一步的分析表明，scg2a缺陷雌性鼠的FeSP神经元没有出现NPBA的积聚、NPBA亚细胞定位的改变或含NPBA的囊泡总数的改变。这表明Scg2a在FeSP神经元中的作用很可能是多效性信号分子的前体。我还分析了缺乏scg2a的青竹的交配行为，发现敲除和野生型个体之间没有差异，这表明scg2a可能对交配行为的调节是可有可无的，或者在调节交配行为方面没有作用。