Vimentin-mediated cellular uptake of C3 exoenzyme

波形蛋白介导的 C3 外切酶的细胞摄取

• 批准号: 323567781
• 负责人: Professorin Dr. Gudrun Ahnert-Hilger
• 金额: --
• 依托单位: Institut für Toxikologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/323567781?language=en
• 关键词: Vimentin; mediated; cellular; uptake; C3

C3bot transferase from Clostridium botulinum and the peptide thereof covering amino acids 154-182 induce axonal and dendritic growth and branching, effects verified on primary neuronal cells in culture as well as in a neuronal lesion animal models. Vimentin, an intermediate filament protein appears to work as the membrane receptor for C3bot. Besides vimentin additional membrane surface molecules (carbohydrates, lipids) may be relevant for C3bot binding and internalization. Using a vimentin knock out (mice) model we confirmed the role of vimentin for C3bot-mediated axonal growth. We now aim to identify and characterize additional cell surface and intracellular receptors relevant for neuronal effects of C3bot. Additionally, we will study the poorly characterized secretion of vimentin and its role in neuronal regeneration.

肉毒梭菌C3bot转移酶及其编码154-182位氨基酸的多肽可诱导轴突和树突的生长和分支，在原代培养的神经细胞上以及在神经元损伤动物模型中得到验证。波形蛋白是一种中间丝蛋白，似乎是C3bot的膜受体。除波形蛋白外，其他膜表面分子(碳水化合物、脂类)可能与C3bot的结合和内化有关。利用波形蛋白敲除(小鼠)模型，我们证实了波形蛋白在C3bot介导的轴突生长中的作用。我们现在的目标是识别和表征与C3bot的神经元效应相关的额外的细胞表面和细胞内受体。此外，我们还将研究波形蛋白的分泌特性不佳及其在神经元再生中的作用。

项目成果

Release of astroglial vimentin by extracellular vesicles: Modulation of binding and internalization of C3 transferase in astrocytes and neurons

• DOI: 10.1002/glia.23566
• 发表时间: 2019-04-01
• 期刊: GLIA
• 影响因子: 6.2
• 作者: Adolf, Andrej;Rohrbeck, Astrid;Hoeltje, Markus
• 通讯作者: Hoeltje, Markus