マイクロヒーター・ナノワイヤを用いた分子交雑に基づくDNA配列解析法の創出

使用微加热器纳米线创建基于分子杂交的DNA序列分析方法

• 批准号: 20J15564
• 负责人: 高橋 博美
• 金额: $ 1.09万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2020
• 资助国家: 日本
• 起止时间: 2020-04-24 至 2022-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20J15564/
• 关键词: nanowires; microfluidic; nucleic acid; ZnO nanowires; Microfluidic

The development of nanowire devices integrated with a microheater (Microheater - ZnO nanowire device) as nucleic acid detection platform using in situ annealed Serinol Nucleic Acid (SNA) probes was demonstrated. First, the microheater could constantly generate suitable heat was confirmed. Second, the use of SNA-MBs to detect target ssDNAs specifically was confirmed. By combining the unique properties of ZnO-NWs and the microheater, the integrated device could detect target ssDNAs using in-situ annealed probes was confirmed. This confirms that this microheater-ZnO-NW device will be applied to fundamental studies related to nanobiodevice-based DNA detection.

展示了使用原位退火丝氨醇核酸（SNA）探针作为核酸检测平台的集成有微加热器的纳米线装置（微加热器- ZnO纳米线装置）的开发。 首先，微加热器可以不断产生适当的热量被证实。 其次，证实了使用SNA-MB特异性地检测靶ssDNA。 通过结合ZnO纳米线和微加热器的独特性能，该集成器件可以使用原位退火探针检测目标ssDNA。 这证实了这种微加热器-ZnO-NW装置将被应用于与基于纳米生物装置的DNA检测相关的基础研究。

项目成果

Nanobiodevices for the Isolation of Circulating Nucleic Acid for Biomedical Applications

用于生物医学应用的循环核酸分离的纳米生物装置

• DOI: 10.1246/cl.210066
• 发表时间: 2021
• 期刊: Chemistry Letters
• 影响因子: 1.6
• 作者: Takahashi Hiromi;Yasui Takao;Baba Yoshinobu
• 通讯作者: Baba Yoshinobu

Microheater-integrated zinc oxide nanowire microfluidic device for hybridization-based detection of target single-stranded DNA

• DOI: 10.1088/1361-6528/abef2c
• 发表时间: 2021-03
• 期刊: Nanotechnology
• 影响因子: 3.5
• 作者: Hiromi Takahashi;T. Yasui;H. Kashida;Koki Makino;Keiko Shinjo;Quanli Liu;Taisuke Shimada;S. Rahong;N. Kaji;H. Asanuma;Y. Baba