Microfluidic nanotechnology for bioanalytical determination of nucleic acid molecules and medicinal compounds

用于核酸分子和药物化合物生物分析测定的微流控纳米技术

基本信息

  • 批准号:
    RGPIN-2022-03320
  • 负责人:
  • 金额:
    $ 2.11万
  • 依托单位:
  • 依托单位国家:
    加拿大
  • 项目类别:
    Discovery Grants Program - Individual
  • 财政年份:
    2022
  • 资助国家:
    加拿大
  • 起止时间:
    2022-01-01 至 2023-12-31
  • 项目状态:
    已结题

项目摘要

Bioanalytical determinations conducted on naturally occurring plants, such as Cannabis (or marijuana) species, will generate knowledge on the identification of the proper species DNA, and on the extraction and quantitation of appropriate medicinal compounds.     The current knowledge gap is to find out appropriate amounts of such compounds obtained from the proper plant species that will generate suitable targeted medicinal and psychedelic effects. For instance, C. indica may consist of more beneficial cannabidiol (CBD) than C. sativa does. The knowledge gap is to be handled by the use of the innovative microfluidic nanotechnology.     The long-term goal of the microfluidic nanotechnology research program is to identify/confirm the proper Cannabis species and detect/quantify their appropriate medicinal compounds.     The short-term objectives over the next five years include nucleic acid testing, and chemical analysis. For nucleic acid testing, the general technique will be amplification tests achieved on plant samples by innovative microfluidic chips for low-volume liquid flow and by gold nanoparticles for molecular or DNA detection. This microfluidic biotechnology enables the molecular testing in order to make the test kits inexpensive and portable for field uses. For chemical analysis of medicinal compounds, the general approach is analytical separations, such as capillary electrophoresis. Using innovative microfluidic nanotechnology, we will integrate sample extraction and analytical separation that enables fast and accurate quantification of medicinal compounds.     For the first time, we obtain preliminary data on detecting DNA based on the coding gene for the enzyme that synthesizes the bioactive compounds. The expected outcomes are to identify the nucleic acids of the Cannabis species and to quantify the plant cannabinoids, such as CBD. Such molecular assays become increasingly important because of agricultural needs and fraud prevention. We are first on quantifying the medicinal compounds in cannabis by conventional analytical separations. We expect to obtain more accurate quantification data enabled by integrating upstream extraction with the microfluidic analytical separation downstream. Such assays are gaining importance recently because of the need of test kits for substance abuse prevention and legal enforcement.     For anticipated impact, the low-cost and portability of test kits are particularly important features for Canadian end users on-site with minimal training. For instance, for the indigenous communities, the test kits are user-friendly; they can analyze their plant samples on-site with a better control, rather than in remote labs that are out of their reach for cross-examinations. The bioanalytical determinations of cannabinoids in plant samples are more accurate when analyzed in the integrated microfluidic system.
对天然存在的植物,如大麻(或大麻)物种进行的生物分析测定将产生关于鉴定适当物种DNA以及提取和定量适当药用化合物的知识。 目前的知识差距是找出从适当的植物物种中获得的适当数量的此类化合物,这些化合物将产生适当的靶向药物和迷幻效果。例如,C. indica可能比C. sativa有。知识差距将通过使用创新的微流体纳米技术来解决。 微流体纳米技术研究计划的长期目标是识别/确认适当的大麻物种,并检测/量化其适当的药用化合物。 未来五年的短期目标包括核酸检测和化学分析。对于核酸检测,一般技术将是通过用于低容量液体流动的创新微流体芯片和用于分子或DNA检测的金纳米颗粒对植物样品进行扩增检测。这种微流体生物技术能够进行分子测试,以使测试试剂盒便宜且便携,可用于现场使用。对于药物化合物的化学分析,一般的方法是分析分离,如毛细管电泳。利用创新的微流体纳米技术,我们将整合样品提取和分析分离,从而实现快速准确的药用化合物定量。 我们首次获得了基于合成生物活性化合物的酶的编码基因检测DNA的初步数据。预期的结果是确定大麻物种的核酸,并量化植物大麻素,如CBD。由于农业需求和欺诈预防,这种分子测定变得越来越重要。我们是第一个通过传统的分析分离来定量大麻中的药用化合物。我们期望通过将上游提取与下游的微流体分析分离相结合来获得更准确的定量数据。由于药物滥用预防和法律的执行需要检测试剂盒,此类测定最近变得越来越重要。 就预期的影响而言,测试包的低成本和便携性对加拿大现场最终用户来说是特别重要的特点,他们只接受了最少的培训。例如,对于土著社区来说,测试工具包是用户友好的;他们可以在现场更好地控制分析他们的植物样本,而不是在他们无法进行交叉检查的偏远实验室。当在集成微流体系统中分析时,植物样品中大麻素的生物分析测定更准确。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Li, Paul其他文献

Li, Paul的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Li, Paul', 18)}}的其他基金

Microfluidic nanotechnology for chemical sensing
用于化学传感的微流控纳米技术
  • 批准号:
    RGPIN-2016-04095
  • 财政年份:
    2021
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Discovery Grants Program - Individual
Microfluidic nanotechnology for chemical sensing
用于化学传感的微流控纳米技术
  • 批准号:
    RGPIN-2016-04095
  • 财政年份:
    2020
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Discovery Grants Program - Individual
Microfluidic nanotechnology for chemical sensing
用于化学传感的微流控纳米技术
  • 批准号:
    RGPIN-2016-04095
  • 财政年份:
    2019
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Discovery Grants Program - Individual
Microfluidic nanotechnology for chemical sensing
用于化学传感的微流控纳米技术
  • 批准号:
    RGPIN-2016-04095
  • 财政年份:
    2018
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Discovery Grants Program - Individual
Microfluidic nanotechnology for chemical sensing
用于化学传感的微流控纳米技术
  • 批准号:
    RGPIN-2016-04095
  • 财政年份:
    2017
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Discovery Grants Program - Individual
Microfluidic nanotechnology for chemical sensing
用于化学传感的微流控纳米技术
  • 批准号:
    RGPIN-2016-04095
  • 财政年份:
    2016
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Discovery Grants Program - Individual
Single rare cell assays and biomarkers detection using microfluidic nanotechnology
使用微流控纳米技术进行单一稀有细胞测定和生物标志物检测
  • 批准号:
    216925-2011
  • 财政年份:
    2015
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Discovery Grants Program - Individual
Single rare cell assays and biomarkers detection using microfluidic nanotechnology
使用微流控纳米技术进行单一稀有细胞测定和生物标志物检测
  • 批准号:
    216925-2011
  • 财政年份:
    2014
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Discovery Grants Program - Individual
Single rare cell assays and biomarkers detection using microfluidic nanotechnology
使用微流控纳米技术进行单一稀有细胞测定和生物标志物检测
  • 批准号:
    216925-2011
  • 财政年份:
    2013
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Discovery Grants Program - Individual
Single rare cell assays and biomarkers detection using microfluidic nanotechnology
使用微流控纳米技术进行单一稀有细胞测定和生物标志物检测
  • 批准号:
    216925-2011
  • 财政年份:
    2012
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Discovery Grants Program - Individual

相似海外基金

Evaluating the impact of spreading depolarisations, post stroke, in the awake brain using graphene-enabled nanotechnology.
使用石墨烯纳米技术评估中风后清醒大脑中扩散去极化的影响。
  • 批准号:
    MR/Y014545/1
  • 财政年份:
    2024
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Research Grant
New nanotechnology for non-invasive deep lung-activatable antimicrobial photodynamic therapy of hospital-associated pneumonia
新型纳米技术用于非侵入性深部肺激活抗菌光动力治疗医院相关性肺炎
  • 批准号:
    478523
  • 财政年份:
    2023
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Operating Grants
Exploring ATR and PARP Inhibitors for Ovarian Cancer Management: An Innovative Approach
探索 ATR 和 PARP 抑制剂用于卵巢癌治疗:一种创新方法
  • 批准号:
    494635
  • 财政年份:
    2023
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Operating Grants
Conference: The 17th US-Korea Forum on Nanotechnology: Next-Generation Semiconductors and the Environmental Implications of Semiconductor Manufacturing
会议:第十七届美韩纳米技术论坛:下一代半导体和半导体制造的环境影响
  • 批准号:
    2309500
  • 财政年份:
    2023
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Standard Grant
Therapeutic nanoscale matrimeres
治疗性纳米级基质
  • 批准号:
    10650665
  • 财政年份:
    2023
  • 资助金额:
    $ 2.11万
  • 项目类别:
Northwestern University O'Brien Kidney National Resource Center
西北大学奥布莱恩肾脏国家资源中心
  • 批准号:
    10754080
  • 财政年份:
    2023
  • 资助金额:
    $ 2.11万
  • 项目类别:
A novel framework for nanomedicine development
纳米医学开发的新框架
  • 批准号:
    10715013
  • 财政年份:
    2023
  • 资助金额:
    $ 2.11万
  • 项目类别:
Influence of Particulate Matter on Fetal Mitochondrial Programming
颗粒物对胎儿线粒体编程的影响
  • 批准号:
    10734403
  • 财政年份:
    2023
  • 资助金额:
    $ 2.11万
  • 项目类别:
New approach based on enzyme stimulating of peptides for targeting drug resistance breast cancers
基于肽酶刺激的新方法用于靶向耐药性乳腺癌
  • 批准号:
    10713648
  • 财政年份:
    2023
  • 资助金额:
    $ 2.11万
  • 项目类别:
Non-invasive detection of tumor NTRK gene fusions via rapid, efficient and low-cost extracellular vesicle isolation method
快速、高效、低成本的细胞外囊泡分离方法无创检测肿瘤NTRK基因融合体
  • 批准号:
    10707684
  • 财政年份:
    2023
  • 资助金额:
    $ 2.11万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了