Elucidating the role of Lrrc34 in the regulation of ribosome biogenesis and Oct4 in mouse and human pluripotent stem cells

在本基金的框架内，将重点分析基因Lrrc34在多能干细胞核仁中的功能。到目前为止，人们对该基因知之甚少：它只在干细胞和男性生殖细胞中表达，其蛋白质主要位于核仁中。研究表明，调控flrrc34的表达可导致胚胎干细胞的差异生长和多能性基因（如Oct4）的表达改变。由于核核是核糖体生物发生的地方，Lrrc34在核糖体生物发生中的作用将被研究。进一步分析Oct4的调控及与Oct4的相互作用。为此，体外试验（小鼠和人类）以及敲除小鼠模型的生成和表征将被使用。

In the frame of the presented grant it will be the focus to analyze thefunction of the gene Lrrc34 in the nucleolus of pluripotent stem cells.Up till know there is not much known about the gene: it is exclusivelyexpressed in stem cells and male germ cells and its protein ispredominantly located in the nucleolus. It is shown, that regulation ofLrrc34 expression resulted in differentially growing of embryonic stemcells and an altered expression of pluripotency genes (e.g. Oct4).Since the nucleolus is the place of ribosome biogenesis,the role of Lrrc34 in ribosome biogenesis will beinvestigated. Furthermore the regulation of and interaction with Oct4 will be further analysed. For this, in vitro assays (mouse and human) as well as the generation and characterization of a knock-out mouse model will be used.