Elucidating the role of Lrrc34 in the regulation of ribosome biogenesis and Oct4 in mouse and human pluripotent stem cells

在本基金的框架下，分析Lrrc 34基因在多能干细胞核仁中的功能将是研究的重点，目前对该基因的了解还不多，它只在干细胞和雄性生殖细胞中表达，其蛋白主要位于核仁中.结果表明，Lrrc34的表达调控导致胚胎干细胞的差异生长和多能性基因（如Oct4）的表达改变，由于核仁是核糖体生物发生的场所，因此Lrrc34在核糖体生物发生中的作用将被研究。此外，还将进一步分析Oct4的调控及其相互作用。为此，将使用体外试验（小鼠和人）以及基因敲除小鼠模型的生成和表征。

In the frame of the presented grant it will be the focus to analyze thefunction of the gene Lrrc34 in the nucleolus of pluripotent stem cells.Up till know there is not much known about the gene: it is exclusivelyexpressed in stem cells and male germ cells and its protein ispredominantly located in the nucleolus. It is shown, that regulation ofLrrc34 expression resulted in differentially growing of embryonic stemcells and an altered expression of pluripotency genes (e.g. Oct4).Since the nucleolus is the place of ribosome biogenesis,the role of Lrrc34 in ribosome biogenesis will beinvestigated. Furthermore the regulation of and interaction with Oct4 will be further analysed. For this, in vitro assays (mouse and human) as well as the generation and characterization of a knock-out mouse model will be used.