Activation of Nucleotides and Amino Acids in Aqueous Media

水介质中核苷酸和氨基酸的活化

• 批准号: 326025951
• 负责人: Professor Dr. Clemens Richert
• 金额: --
• 依托单位: Institut für Organische Chemie (IOC)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/326025951?language=en
• 关键词: Activation; Nucleotides; Amino; Acids; Aqueous

Reactions of activated ribonucleotides and other biomolecules in aqueous solution are pivotal for the cell. Triphosphates, such as ATP or GTP induce the majority of endergonic reactions of primary metabolism. At the same time, ATP, GTP, CTP, and UTP are also monomers for the synthesis of RNA strands in all known forms of life. Finally, reactions of amino acids and ATP are critical for the biosynthesis of polypeptides. During our study of enzyme-free primer extensions with in situ activation we identified reaction conditions that induce the simultaneous growth of RNA and peptide chains, starting from free amino acids and ribonucleotides in aqueous buffer. The available data suggest that activated nucleotides play a key role in these processes. Gaining a better understanding of the underlying activation chemistry is the focus of the project. This activation chemistry appears to produce an organically activated species reminiscent of ATP in biochemistry. In this context, the organocatalysts inducing efficient chain growth will be studied.

在水溶液中，活化的核糖核苷酸和其他生物分子的反应对细胞至关重要。三磷酸盐，如ATP或GTP诱导初级代谢的大多数吸能反应。同时，ATP、GTP、CTP和UTP也是所有已知生命形式中合成RNA链的单体。最后，氨基酸和ATP的反应对于多肽的生物合成至关重要。在我们的研究中，无酶引物延伸与原位活化，我们确定了反应条件，诱导RNA和肽链的同时增长，从游离氨基酸和核糖核苷酸在水性缓冲液。现有的数据表明，活化的核苷酸在这些过程中发挥关键作用。更好地理解潜在的活化化学是该项目的重点。这种活化化学似乎产生一种有机活化的物质，让人想起生物化学中的ATP。在这方面，有机催化剂诱导有效的链增长将进行研究。

项目成果

Prebiotic chemistry and human intervention

生命起源前的化学和人类干预

• DOI: 10.1038/s41467-018-07219-5
• 发表时间: 2018
• 期刊: Nature Communications
• 影响因子: 16.6
• 作者: C. Richert

Regioselective formation of RNA strands in the absence of magnesium ions

• DOI: 10.1093/nar/gkz1125
• 发表时间: 2020-02-20
• 期刊: NUCLEIC ACIDS RESEARCH
• 影响因子: 14.9
• 作者: Motsch, Sebastian;Tremmel, Peter;Richert, Clemens
• 通讯作者: Richert, Clemens

Enzyme-free ligation of dimers and trimers to RNA primers

• DOI: 10.1093/nar/gkz160
• 发表时间: 2019-05-07
• 期刊: NUCLEIC ACIDS RESEARCH
• 影响因子: 14.9
• 作者: Sosson, Marilyne;Pfeffer, Daniel;Richert, Clemens
• 通讯作者: Richert, Clemens