Budding and asymmetric cell division in Hyphomonas neptunium (P04)

Hyphomonas neptunium (P04) 的出芽和不对称细胞分裂

• 批准号: 326464661
• 金额: --
• 依托单位: Arbeitsgruppe Prokaryotische Zellbiologie
• 依托单位国家: 德国
• 项目类别: CRC/Transregios
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/326464661?language=en
• 关键词: Budding; asymmetric; cell; division; Hyphomonas

In bacteria, ParB homologs play a central role in the partitioning of replicated DNA to the daughter cells. However, the precise mechanism underlying their function still remains unclear. Recent work from our group and others revealed that members of this protein family bind and hydrolyze the ribonucleotide CTP to bind reversibly to DNA, thus constituting a new class of nucleotide-depen¬dent molecular switches. In this project, we build on these advances and aim to obtain a compre¬hensive and quantitative mechanistic understanding of the role of ParB in chro-mosome seg¬regation and the coordination of this process with cell division. Moreover, we will set out to identify thus-far uncharac¬ter¬ized proteins that share the switch-like properties of ParB but control cellular pro¬cesses different from DNA segregation. Together, these studies will provide detailed insight into the role of CTP-dependent molecular switches in bacterial cell biology.

在细菌中，PARB同源物在将复制的DNA分配给子细胞中起着核心作用。但是，其功能的基础机制仍然不清楚。我们小组和其他人的最新工作表明，该蛋白质家族的成员结合并水解了核糖核苷酸CTP，以可逆地与DNA结合，从而构成了一类新的核苷酸 - 二培养基分子开关。在这个项目中，我们以这些进步为基础，并旨在获得对PAR在CHRO-MOSOMOSOMOMOSOMESEDRICATION中的作用以及该过程与细胞分裂的协调的全面和定量的机械理解。此外，我们将着手识别出合理的蛋白质，这些蛋白质具有共享PARB的开关性特性，但控制细胞促进与DNA分离不同。总之，这些研究将提供详细的见解，了解CTP依赖性分子开关在细菌细胞生物学中的作用。