Budding and asymmetric cell division in Hyphomonas neptunium (P04)

Hyphomonas neptunium (P04) 的出芽和不对称细胞分裂

• 批准号: 326464661
• 金额: --
• 依托单位: Arbeitsgruppe Prokaryotische Zellbiologie
• 依托单位国家: 德国
• 项目类别: CRC/Transregios
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/326464661?language=en
• 关键词: Budding; asymmetric; cell; division; Hyphomonas

In bacteria, ParB homologs play a central role in the partitioning of replicated DNA to the daughter cells. However, the precise mechanism underlying their function still remains unclear. Recent work from our group and others revealed that members of this protein family bind and hydrolyze the ribonucleotide CTP to bind reversibly to DNA, thus constituting a new class of nucleotide-depen¬dent molecular switches. In this project, we build on these advances and aim to obtain a compre¬hensive and quantitative mechanistic understanding of the role of ParB in chro-mosome seg¬regation and the coordination of this process with cell division. Moreover, we will set out to identify thus-far uncharac¬ter¬ized proteins that share the switch-like properties of ParB but control cellular pro¬cesses different from DNA segregation. Together, these studies will provide detailed insight into the role of CTP-dependent molecular switches in bacterial cell biology.

在细菌中，ParB同系物在将复制的DNA分配到子细胞中发挥核心作用。然而，其功能背后的确切机制仍不清楚。本课题组和其他研究人员最近的工作表明，该蛋白质家族的成员结合并水解核糖核苷酸CTP以可逆地结合DNA，从而构成一类新的核苷酸依赖性分子开关。在这个项目中，我们建立在这些进展的基础上，旨在获得对ParB在染色体分离中的作用以及该过程与细胞分裂的协调的全面和定量的机制理解。此外，我们将着手鉴定迄今为止未知的蛋白质，其具有ParB的开关样性质，但控制不同于DNA分离的细胞过程。总之，这些研究将为CTP依赖性分子开关在细菌细胞生物学中的作用提供详细的见解。