Z-Project (coordination and central funds)

Z-项目（协调和中央资金）

• 批准号: 32972272
• 负责人: Professor Dr. Torsten Schöneberg
• 金额: --
• 依托单位: Rudolf-Boehm-Institut für Pharmakologie und Toxikologie
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/32972272?language=en
• 关键词: Project; coordination; central; funds

P2 receptors in the peripheral and central nervous system are targets of extracellular nucleotides. Especially ATP/ADP and UTP/UDP are important signalling molecules. Our consortium will investigate ligand-gated P2X and G protein-coupled P2Y receptors at neurons, glial cells and their common precursor cells. We intend to elucidate the structure, assembly, transduction pathways and interaction mechanisms of these receptors. The structural pre-requisites for ligand-binding or agonist promiscuity will be clarified. Transgenic mice will be generated to facilitate the visual identification and mapping of the relevant receptor classes as well as to study their up- or down-regulation and their accessory proteins. We will also carry out functional studies on a few selected cell systems (sensory neurons, astrocytes, Müller cells, microglia) where P2X and/or P2Y receptors may play important roles in the development of e.g. neuropathic pain, retinal damage, Parkinson¿s disease or neurodegenerative illnesses. A wide methodological competence is available to approach the targeted aims. The already mentioned transgenic animals generated by the members of the Research Unit and additional knockout mice obtained from external sources will be used collectively. The projects will either be carried out at the Medical Faculty of the University of Leipzig or they will be intimately associated with this Faculty. It is intended to establish in Leipzig a nationwide center for coordinating German P2 receptor research.

外周和中枢神经系统中的P2受体是细胞外核苷酸的靶点。尤其是ATP/ADP和UTP/UDP是重要的信号分子。我们的联盟将研究配体门控的P2 X和G蛋白偶联的P2 Y受体在神经元，神经胶质细胞及其共同的前体细胞。本文旨在阐明这些受体的结构、组装、转导途径和相互作用机制。将阐明配体结合或激动剂混杂的结构前体。将产生转基因小鼠，以促进相关受体类别的视觉识别和映射，以及研究它们的上调或下调及其辅助蛋白。我们还将对一些选定的细胞系统（感觉神经元，星形胶质细胞，Müller细胞，小胶质细胞）进行功能研究，其中P2 X和/或P2 Y受体可能在神经性疼痛，视网膜损伤，帕金森病或神经退行性疾病的发展中发挥重要作用。有广泛的方法能力来实现既定目标。将共同使用研究单位成员产生的上述转基因动物和从外部来源获得的额外敲除小鼠。这些项目将在莱比锡大学医学院进行，或者与该学院密切相关。计划在莱比锡建立一个协调德国P2受体研究的全国性中心。