インタラクトーム解析に向けた高速多色振動分光イメージング

今年，我们主要致力于超多路生物成像的主题，主要是朝着超多路超分辨率成像方向发展。通过应用之前发展的光开关受激拉曼散射显微技术，我们成功地展示了具有高化学特异性的超分辨率受激拉曼散射成像。同时，新开发的可光开关拉曼探针使我们能够针对特定类型的细胞内细胞器进行有意义的生物成像。评价的空间分辨率突破了振动成像情况下的衍射极限。窄线宽和超分辨特性都表明，所开发的技术是迈向最终超多路超分辨成像的重要一步。

This year, we have worked on the theme of ‘ super-multiplex biological imaging’ mainly towards super-multiplex super-resolution imaging. Via applying the technology of photoswitchable stimulated Raman scattering microscopy developed before which is also a part of this project, we successfully demonstrate super-resolution stimulated Raman scattering imaging with high chemical specificity. Meanwhile, the newly developed photoswitchable Raman probe enable us to target certain kind of intracellular organelle for meaningful biological imaging. The evaluated spatial resolution has broken the diffraction limitation in the circumstance of vibrational imaging. Both the narrow linewidth and super-resolution property shows the developed technique as an important step towards final super-multiplex super-resolution imaging.