インタラクトーム解析に向けた高速多色振動分光イメージング

用于相互作用组分析的高速多色振动光谱成像

• 批准号: 19J22546
• 负责人: 寿 景文
• 金额: $ 1.6万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2019
• 资助国家: 日本
• 起止时间: 2019-04-25 至 2022-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-19J22546/
• 关键词: super-multiplex; super-resolution; stimulated Raman; fluorescence; high-speed; Super-multiplex; Stimulated Raman; Fluorescence; High-speed

This year, we have worked on the theme of ‘ super-multiplex biological imaging’ mainly towards super-multiplex super-resolution imaging. Via applying the technology of photoswitchable stimulated Raman scattering microscopy developed before which is also a part of this project, we successfully demonstrate super-resolution stimulated Raman scattering imaging with high chemical specificity. Meanwhile, the newly developed photoswitchable Raman probe enable us to target certain kind of intracellular organelle for meaningful biological imaging. The evaluated spatial resolution has broken the diffraction limitation in the circumstance of vibrational imaging. Both the narrow linewidth and super-resolution property shows the developed technique as an important step towards final super-multiplex super-resolution imaging.

今年，我们主要致力于超多路生物成像的主题，主要是朝着超多路超分辨率成像方向发展。通过应用之前发展的光开关受激拉曼散射显微技术，我们成功地展示了具有高化学特异性的超分辨率受激拉曼散射成像。同时，新开发的可光开关拉曼探针使我们能够针对特定类型的细胞内细胞器进行有意义的生物成像。评价的空间分辨率突破了振动成像情况下的衍射极限。窄线宽和超分辨特性都表明，所开发的技术是迈向最终超多路超分辨成像的重要一步。

项目成果

Multicolor stimulated Raman and fluorescence imaging for investigating lipid metabolism

用于研究脂质代谢的多色受激拉曼和荧光成像

• 发表时间: 2019
• 作者: J. Shou;Y. Ozeki;Robert Oda;Jingwen Shou;Jingwen Shou;Jingwen Shou
• 通讯作者: Jingwen Shou

Axicon-based beam shaping for low-loss nonlinear microscopic optics

用于低损耗非线性显微光学的基于轴棱镜的光束整形

• DOI: 10.1364/josab.36.001342
• 发表时间: 2019
• 期刊: Journal of the Optical Society of America B
• 作者: Ochiai Natsuha;Shou Jingwen;Ozeki Yasuyuki
• 通讯作者: Ozeki Yasuyuki

Columbia University(米国)

哥伦比亚大学（美国）

Activatable型ラマンプローブ

可激活拉曼探头

• 发表时间: 2019

Multicolor Activatable Raman Probes for Simultaneous Detection of Plural Enzyme Activities

• DOI: 10.1021/jacs.0c09200
• 发表时间: 2020-12-09
• 期刊: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
• 影响因子: 15
• 作者: Fujioka, Hiroyoshi;Shou, Jingwen;Kamiya, Mako
• 通讯作者: Kamiya, Mako