Supramolecular interactions between proteins and bioadhesive nanoparticles

蛋白质和生物粘附纳米粒子之间的超分子相互作用

基本信息

批准号：
19J23043
负责人：
Morishita Kiyoshi
金额：
$ 2.18万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for JSPS Fellows
财政年份：
2019
资助国家：
日本
起止时间：
2019-04-25 至 2022-03-31
项目状态：
已结题

项目摘要

This year I worked on i) porous clusters formed by GroEL and gold nanoparticles (AuNPs), and ii) rugby ball self-assembly of DNA-functionalized GroEL.In project i) I confirmed the ATPase activity of the clusters and analyzed the porous nature of the clusters by TEM, cryo TEM, and cryo-EM tomography. This quantitative approach confirmed that the clusters had discrete numbers of GroEL connecting AuNPs, as designed. The porous nature was also probed by guest loading studies, using various polymer-protein hybrids as guest species. This indicated that the cluster can accommodate guests on the order of several tens of nanometers in size, with a guest hydrodynamic diameter limit of around 45 nm. Experiments on the effect of guest polymer conformation on loading are ongoing.In project ii) I studied the roles of various nucleotides and analogues, DNA sequence, and salt conditions in the rugby ball formation. This gave a complete understanding of the important factors for growth and supported a proposed mechanism for the assembly. I identified the alignment of GroEL units in the structure and its anisotropic nature. Current experiments are targeted to identify whether the structure is hollow.

今年，我研究了由凹槽和金纳米粒子（AUNPS）组成的多孔簇，以及ii）DNA功能化的groel的橄榄球球自组装。在项目i）我确认了簇的ATPase活性，并通过Tem，Cryo cryo tem tem和Cryo-em-em smograph进行了分析簇的ATPase活性。这种定量方法证实了该簇具有分散数量的连接aunps的凹槽。宾客装载研究还使用各种聚合物 - 蛋白质杂种作为宾客物种来探测多孔性质。这表明该群集可以以几十纳米的大小为客人容纳客人，并且客人的流体动力直径极限约为45 nm。关于来宾聚合物构象对负载的影响的实验。这完全了解了增长的重要因素，并支持了组装的提议机制。我确定了结构及其各向异性性质中的凹槽单元的对齐。当前的实验是针对确定结构是否是空心的。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Programmed Porous Nanostructures with Biomolecular Machines and Nanoparticles

用生物分子机器和纳米颗粒编程多孔纳米结构

DOI：
发表时间：
2022
期刊：
影响因子：
0
作者：
Kiyoshi Morishita;P.K. Hashim;Haruaki Yanagisawa;Masahide Kikkawa;Tatsuya Niwa;Hideki Taguchi;Takuzo Aida
通讯作者：
Takuzo Aida

University of Santiago de Compostela(スペイン)

圣地亚哥德孔波斯特拉大学（西班牙）