Supramolecular interactions between proteins and bioadhesive nanoparticles

蛋白质和生物粘附纳米粒子之间的超分子相互作用

• 批准号: 19J23043
• 负责人: Morishita Kiyoshi
• 金额: $ 2.18万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2019
• 资助国家: 日本
• 起止时间: 2019-04-25 至 2022-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-19J23043/
• 关键词: Supramolecular chemistry; Colloidal Assembly; DNA nanotechnology; Nanoparticles; Proteins; GroEL; Materials Science; Colloidal assembly; Nanobiotechnology; Materials Engineering; nanoparticle; protein; DNA; supramolecular chemistry; assembly

This year I worked on i) porous clusters formed by GroEL and gold nanoparticles (AuNPs), and ii) rugby ball self-assembly of DNA-functionalized GroEL.In project i) I confirmed the ATPase activity of the clusters and analyzed the porous nature of the clusters by TEM, cryo TEM, and cryo-EM tomography. This quantitative approach confirmed that the clusters had discrete numbers of GroEL connecting AuNPs, as designed. The porous nature was also probed by guest loading studies, using various polymer-protein hybrids as guest species. This indicated that the cluster can accommodate guests on the order of several tens of nanometers in size, with a guest hydrodynamic diameter limit of around 45 nm. Experiments on the effect of guest polymer conformation on loading are ongoing.In project ii) I studied the roles of various nucleotides and analogues, DNA sequence, and salt conditions in the rugby ball formation. This gave a complete understanding of the important factors for growth and supported a proposed mechanism for the assembly. I identified the alignment of GroEL units in the structure and its anisotropic nature. Current experiments are targeted to identify whether the structure is hollow.

今年，我研究了 i) 由 GroEL 和金纳米粒子 (AuNPs) 形成的多孔团簇，以及 ii) DN​​A 功能化 GroEL 的橄榄球自组装。在项目 i) 中，我确认了团簇的 ATPase 活性，并通过 TEM、冷冻 TEM 和冷冻电镜断层扫描分析了团簇的多孔性质。这种定量方法证实了簇具有离散数量的 GroEL 连接 AuNP，正如设计的那样。还通过客体负载研究探索了多孔性质，使用各种聚合物-蛋白质杂化物作为客体物种。这表明该簇可以容纳尺寸为几十纳米的客体，客体流体动力学直径极限约为 45 nm。关于客体聚合物构象对负载影响的实验正在进行中。在项目 ii) 中，我研究了各种核苷酸和类似物、DNA 序列以及盐条件在橄榄球形成中的作用。这使人们对增长的重要因素有了全面的了解，并支持了拟议的大会机制。我确定了结构中 GroEL 单元的排列及其各向异性性质。目前的实验旨在确定该结构是否是空心的。

项目成果

Programmed Porous Nanostructures with Biomolecular Machines and Nanoparticles

用生物分子机器和纳米颗粒编程多孔纳米结构

• 发表时间: 2022
• 作者: Kiyoshi Morishita;P.K. Hashim;Haruaki Yanagisawa;Masahide Kikkawa;Tatsuya Niwa;Hideki Taguchi;Takuzo Aida
• 通讯作者: Takuzo Aida

University of Santiago de Compostela(スペイン)

圣地亚哥德孔波斯特拉大学（西班牙）

University of Hamburg(ドイツ)

汉堡大学（德国）

Multifunctional Gold Nanoparticles for Protein Degradation

用于蛋白质降解的多功能金纳米颗粒

• 发表时间: 2018
• 作者: Kiyoshi Morishita;Kou Okuro;Takuzo Aida
• 通讯作者: Takuzo Aida

Vilnius University(リトアニア)

维尔纽斯大学（立陶宛）