Microbial desulfonation pathways for natural and pharmacologically relevant C3 sulfonates

天然和药理学相关的 C3 磺酸盐的微生物脱磺化途径

• 批准号: 33360182
• 负责人: Professor Dr. Alasdair M. Cook
• 金额: --
• 依托单位: Arbeitsgruppe Mikrobielle Ökologie (aufgelöst)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2009-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/33360182?language=en
• 关键词: Microbial; desulfonation; pathways; natural; pharmacologically

C3 sulfonates are both natural compounds and xenobiotics, examples of which are subject to bacterial degradation. Widespread natural products are L-cysteate (3-sulfo-2-aminopropionate) and sulfolactate where an initial understanding of the bacterial desulfonation is available. Homotaurine (3-aminopropanesulfonate) and its derivatives are (i) pharmacologically relevant in the treatement of Alzheimer´s disease, stroke or alcoholism, and (ii) widly used as surfactants or buffers in chemistry. Little is known about the presumably oxygenolytic desulfonation of homotaurine and the related propane-1,3-disulfonate. A project for a PhD thesis involves physiological, biochemical and genetic methods to establish complete degradative pathways for these synthetic C3 sulfonates. Homotaurine is used by bacteria as a carbon or as a nitrogen source, and different degradative pathways are anticipated. We are most interested in the desulfonative enzymes, the corresponding genes, their regulation and in identifying the transporters. Two extensive HiWi projects will expand our knowledge on the degradation of the natural C3 sulfonates and explore the first known desulfonation reaction in an archeon.

C3磺酸盐是天然化合物和异生物质，其实例易受细菌降解。广泛存在的天然产物是L-半胱氨酸（3-磺基-2-氨基丙酸酯）和磺基乳酸，其中对细菌磺化有初步了解。高牛磺酸（3-氨基丙磺酸盐）及其衍生物（i）与阿尔茨海默病、中风或酒精中毒的缓解密切相关，（ii）在化学中广泛用作表面活性剂或缓冲剂。关于高牛磺酸和相关的丙烷-1，3-二磺酸盐的可能的氧解磺化作用知之甚少。博士论文的一个项目涉及生理学，生物化学和遗传学方法，以建立这些合成C3磺酸盐的完整降解途径。高牛磺酸被细菌用作碳源或氮源，并且预期有不同的降解途径。我们最感兴趣的是代谢酶，相应的基因，它们的调控和识别转运蛋白。两个广泛的HiWi项目将扩大我们对天然C3磺酸盐降解的了解，并探索第一个已知的在archeon中的磺化反应。