Microbial generation and dissimilation of isethionate and sulfoacetate: enzymes, genes, transporter genes and regulation

微生物产生羟乙基磺酸盐和磺基乙酸盐的异化：酶、基因、转运蛋白基因和调控

• 批准号: 19964677
• 负责人: Professor Dr. Alasdair M. Cook
• 金额: --
• 依托单位: Arbeitsgruppe Mikrobielle Ökologie (aufgelöst)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2005
• 资助国家: 德国
• 起止时间: 2004-12-31 至 2009-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/19964677?language=en
• 关键词: Microbial; generation; dissimilation; isethionate; sulfoacetate

The C2-sulfonates isethionate (2-hydroxyethanesulfonate) and sulfoacetate are widespread natural products; they are also produced industrially for use in skincare products and the formulation of pharmaceuticals. Despite their significant abundance, neither the degradation nor the formation of these compounds by bacteria has been elucidated in detail. Initial studies related to work on the degradative pathway for taurine (2-aminoethanesulfonate) show that all pathways share a key intermediate, sulfoacetaldehyde. The initial enzymes leading from isethionate and sulfoacetate to this intermediate are unknown. Further work with taurine, as a nitrogen source, showed the generation and excretion of isethionate or sulfoacetate. This project will elucidate the microbial biochemistry of the degradation of isethionate and sulfoacetate, on the one hand, and of the production of these compounds on the other. Reverse genetics will allow the corresponding genes and flanking regions to be identified and sequenced, though some surroundings should be available from genome sequencing projects. Given clusters of genes in taurine degradation, we anticipate clusters in the pathways under study. The clusters should allow candidates for transport systems, regulators and exporters of sulfo-oxyanions to be identified and characterized.

C2-磺酸盐、异硫酸盐(2-羟基乙基磺酸盐)和磺酸乙酯是广泛存在的天然产物；它们也在工业上生产，用于护肤产品和药物配方。尽管它们的含量非常丰富，但细菌对这些化合物的降解或形成都没有详细阐明。与牛磺酸(2-氨基乙磺酸盐)降解途径相关的初步研究表明，所有途径都共享一个关键的中间体-磺乙醛。从异硫酸盐和磺酸乙酸酯到这个中间体的起始酶是未知的。用牛磺酸作为氮源的进一步研究表明，异硫酸盐或磺酸乙酸酯的生成和排泄。该项目一方面将阐明异硫酸盐和磺酸乙酯降解的微生物生物化学，另一方面将阐明这些化合物的产生。反向遗传学将允许识别和测序相应的基因和侧翼区域，尽管一些环境应该可以从基因组测序项目中获得。给出牛磺酸降解的基因簇，我们预计在研究的途径中会有簇。集群应使运输系统、调节器和磺氧阴离子出口商的候选者得以确定和确定其特征。