Investigation of the role of the homologous proteins PCH1 and PCHL in phytochrome B-mediated light signalling

同源蛋白 PCH1 和 PCHL 在光敏色素 B 介导的光信号传导中的作用研究

• 批准号: 336146752
• 负责人: Professor Dr. Andreas Hiltbrunner
• 金额: --
• 依托单位: Institut für Biologie II
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/336146752?language=en
• 关键词: Investigation; role; homologous; proteins; PCH1

Phytochromes are photoreceptors in plants that regulate growth and development in response to changes in the environment. Light quality and intensity control the conversion from the inactive Pr to the active Pfr state as well as from Pfr back to Pr. In addition, reversion of Pfr to Pr also occurs in a light-independent process called dark reversion. Phytochrome B (phyB) is of particular importance in light-grown and adult plants and plays a role in detection of light in the red light range of the visible spectrum. We recently identified PCH1 and PCHL as phyB binding proteins. Plants overexpressing PCH1 or PCHL are strongly hypersensitive to red light, while lack of functional PCH1 results in reduced responses to red light. Initial experiments show that PCH1 and PCHL decrease phyB dark reversion and thereby maintain high Pfr levels. In the proposed project, we want to investigate molecular mechanisms underlying PCH1/PCHL-mediated regulation of phyB activity, with particular emphasis on PCH1/PCHL-dependent control of dark reversion. We also want to investigate a potential role of PCH1/PCHL in cross-talk between phyB and other signalling pathways, which could affect the activity of phyB by regulation of PCH1/PCHL. Our own data and data in the literature suggest that PCH1/PCHL link other light signalling pathways to phyB-mediated red light signalling. Moreover, PCH1/PCHL might play a role during seed germination by increasing the light sensitivity of imbibed seeds and provide a molecular link between phyB and karrikins, which are chemical compounds in smoke that promote germination and seedling development. PhyB dark reversion is temperature-dependent and therefore phyB might also have a function as temperature sensor. Given that PCH1/PCHL affect dark reversion, they might play a role in temperature sensing, which we also want to investigate.

光敏色素是植物中的光感受器，其调节生长和发育以响应环境的变化。光的质量和强度控制的转换，从非活性Pr到活性Pfr状态，以及从Pfr回Pr。此外，逆转Pfr Pr也发生在一个光独立的过程称为黑暗逆转。光敏色素B（phyB）在光生长和成株植物中特别重要，并且在检测可见光谱的红光范围内的光中起作用。我们最近发现PCH 1和PCHL作为phyB结合蛋白。过表达PCH 1或PCHL的植物对红光非常敏感，而功能性PCH 1的缺乏导致对红光的反应降低。初步实验表明，PCH 1和PCHL减少phyB暗回复，从而保持高Pfr水平。在拟议的项目中，我们希望研究潜在的PCH 1/PCHL介导的phyB活性调节的分子机制，特别强调PCH 1/PCHL依赖的黑暗逆转控制。我们还希望研究PCH 1/PCHL在phyB和其他信号通路之间的串扰中的潜在作用，这可能通过调节PCH 1/PCHL来影响phyB的活性。我们自己的数据和文献中的数据表明，PCH 1/PCHL连接其他光信号通路的phyB介导的红光信号。此外，PCH 1/PCHL可能在种子萌发过程中发挥作用，通过增加吸胀种子的光敏感性，并提供phyB和karrikins之间的分子联系，这是烟雾中的化学化合物，促进发芽和幼苗发育。PhyB暗回复是温度依赖性的，因此PhyB也可能具有温度传感器的功能。鉴于PCH 1/PCHL影响暗回复，它们可能在温度传感中发挥作用，我们也想研究这一点。