Using natural variation for the investigation of the phytochrome A sequence/function relationship

利用自然变异研究光敏色素 A 序列/功能关系

• 批准号: 445757564
• 负责人: Professor Dr. Andreas Hiltbrunner
• 金额: --
• 依托单位: Institut für Biologie II
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/445757564?language=en
• 关键词: Using; natural; variation; investigation; phytochrome

Plants use various classes of photoreceptors to measure diverse aspects of their ambient environment. By converting light spectra into transducible signals, these receptors control nuclear gene expression to modify growth in response to diverse stimuli such as spectral composition and light intensity. Phytochrome A is the only far-red light receptor in plants and plays a critical role for germination and seedling establishment in deep canopy shade. In the proposed project, we aim to find amino acid polymorphisms which alter a plant’s capacity to respond to far-red light and describe how they affect the ability of phytochrome A to function as a protein scaffold upon which diverse light dependent pathways are integrated. To this end, we will screen the 1135 Arabidopsis accessions of the 1001 Genomes Project for accessions that are compromised in their ability to respond to far-red light and pinpoint characteristic changes in the primary sequences of phytochrome A. We then test the effects these amino acid substitutions have on protein-protein interactions involving phytochrome A and characterise the properties of the different phytochrome A variants from accessions with altered response to far-red light. The goal of the project will be to establish a set of important amino acid substitutions and link them to the protein-protein interactions and other critical properties of phytochrome A which they influence.

植物使用各种类型的光感受器来测量其周围环境的各个方面。通过将光谱转化为可转导的信号，这些受体控制核基因表达，以响应不同的刺激，如光谱组成和光强度，改变生长。光敏色素A是植物体内唯一的远红光受体，对植物在遮荫条件下的萌发和幼苗形成起着重要作用。在拟议的项目中，我们的目标是找到改变植物对远红光的反应能力的氨基酸多态性，并描述它们如何影响光敏色素A作为蛋白质支架的能力，在该蛋白质支架上整合了多种光依赖性途径。为此，我们将筛选1001基因组计划的1135份拟南芥种质，寻找对远红光反应能力受损的种质，并确定光敏色素A一级序列的特征变化。然后，我们测试这些氨基酸取代对蛋白质-蛋白质相互作用的影响，包括光敏色素A和光敏色素A的不同变体的性质，从加入改变响应远红光。该项目的目标是建立一套重要的氨基酸取代，并将其与蛋白质-蛋白质相互作用和它们影响的光敏色素A的其他关键特性联系起来。