Comparative Analysis of the Stage- and Species-Specificity of Rust Secretomes.

锈病分泌物阶段和物种特异性的比较分析。

• 批准号: 34569014
• 负责人: Professor Dr. Ralf Thomas Vögele
• 金额: --
• 依托单位: Fachgebiet Phytopathologie (360a)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/34569014?language=en
• 关键词: Comparative; Analysis; Stage; Species; Specificity

Obligate biotrophic fungi are characterized by a prolonged co-existence of host and parasite. It is highly likely that proteins, so called effectors, are involved in establishing and maintaining this intricate interaction. A hallmark of obligate biotrophs is the formation of specially differentiated hyphae, so called haustoria, penetrating into the host cell. These haustoria are the ideal place for a transfer of effectors into the interface between host and parasite or even into the host cell itself.The haustorial secretome of Uromyces fabae was analyzed and compared to the secretome of in vitro grown infection structures representing developmental stages prior to the parasitic stage. We found 62 genes encoding proteins secreted from haustoria and 42 genes encoding proteins secreted from in vitro grown infection structures. Four of these genes were present in both libraries. These results indicate a strong stage-specificity of protein secretion in rusts. Similarities were found for 39 of the sequences, 28 of which showed similarity to proteins identified among other rusts only. This was taken as evidence for roles of these proteins in virulence/pathogenicity and host specificity unique to the Uredinales. Selected proteins were localized immunocytologically and searches for interaction partners have been initiated. One candidate effector protein, Uf- HSP25p, could be localized within the plant cell nucleus. Both experimental approaches will be further pursued. During the second phase of the project we intend to analyze the haustorial secretomes of Phakopsora pachyrhizi and Uromyces appendiculatus by using the yeast signal sequence trap established with U. fabae. This will create opportunities to compare the different secretomes and identify secreted proteins essential for virulence/pathogenicity.

专性生物营养真菌的特征是宿主和寄生虫长期共存。蛋白质（所谓的效应子）很可能参与建立和维持这种复杂的相互作用。专性生物营养生物的一个标志是形成特殊分化的菌丝，即所谓的吸器，渗透到宿主细胞中。这些吸器是将效应器转移到宿主和寄生虫之间的界面，甚至转移到宿主细胞本身的理想场所。对蚕豆泌尿菌的吸器分泌组进行了分析，并与代表寄生阶段之前发育阶段的体外生长的感染结构的分泌组进行了比较。我们发现了 62 个编码吸器分泌蛋白的基因和 42 个编码体外生长的感染结构分泌蛋白的基因。其中四个基因均存在于两个文库中。这些结果表明锈病中蛋白质分泌具有很强的阶段特异性。发现了 39 个序列的相似性，其中 28 个序列与仅在其他锈菌中鉴定出的蛋白质相似。这被视为这些蛋白质在毒力/致病性和乌雷​​丁目特有的宿主特异性中的作用的证据。选定的蛋白质通过免疫细胞学定位，并开始寻找相互作用伙伴。一种候选效应蛋白 Uf-HSP25p 可能位于植物细胞核内。这两种实验方法都将得到进一步推行。在该项目的第二阶段，我们打算通过使用豆豆菌建立的酵母信号序列陷阱来分析豆薯层锈菌和阑尾囊霉的吸器分泌体。这将为比较不同的分泌蛋白组并鉴定毒力/致病性所必需的分泌蛋白创造机会。