Transcription factor – enhancer – promoter based regulatory networks

转录因子→增强子→基于启动子的调控网络

• 批准号: DP160101960
• 负责人: Prof Alistair Forrest
• 金额: $ 32.07万
• 依托单位: The University of Western Australia
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2016
• 资助国家: 澳大利亚
• 起止时间: 2016-06-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP160101960
• 关键词: Transcription; factor; enhancer; promoter; based

This project aims to develop new understanding on how multicellular organisms (including humans) develop, and how mutations in distant regions of the genome can affect human traits. The way the human genome is interpreted by the cellular machinery is still a mystery. We have a reference sequence and know where the majority of coding genes are, but we are far from understanding how the genome is regulated to generate the diversity of cell types in our bodies. Enhancer regions interact with proximal promoters to regulate gene expression level and tissue-specificity. This project aims to develop transcriptional regulatory network models using high throughput chromatin interaction data and expression perturbation to link promoter and enhancers genome-wide.

该项目旨在发展对多细胞生物（包括人类）如何发展的新认识，以及基因组遥远区域的突变如何影响人类特征。细胞机制解释人类基因组的方式仍然是一个谜。我们有一个参考序列，知道大多数编码基因在哪里，但我们还远远不了解基因组是如何被调节的，从而在我们体内产生细胞类型的多样性。增强子区域与近端启动子相互作用，调节基因表达水平和组织特异性。该项目旨在利用高通量染色质相互作用数据和表达扰动建立转录调控网络模型，以连接全基因组的启动子和增强子。