Partitioning of sulfur between primary and secondary metabolism

硫在初级代谢和次级代谢之间的分配

• 批准号: 372353760
• 负责人: Professor Dr. Stanislav Kopriva
• 金额: --
• 依托单位: Arbeitsgruppe Kopriva
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/372353760?language=en
• 关键词: Partitioning; sulfur; between; primary; secondary

Sulfur is an essential nutrient in plants, present in a range of essential primary metabolites as well as in numerous secondary compounds. Due to its chemistry, sulfur in the primary metabolites is usually incorporated in a reduced form as a thiol or sulfide, whereas the secondary compounds contain often oxidized sulfur as organic sulfate. We have characterized 4 isoforms of the enzyme adenosine 5´-phosphosulfate (APS) kinase in Arabidopsis as being essential for completion of the life cycle and for synthesis of sulfated secondary products, such as glucosinolates. Our analyses however left several important questions unanswered: (i.) What is the role of redox regulation of APS kinase for control of partitioning of S between primary and secondary metabolism (ii.) Why is APS kinase isoform APK2 alone not capable to sustain growth of Arabidopsis, and (iii.) What is the importance of APS kinase in plant species not producing glucosinolates? This proposal addresses these questions in order to obtain more detailed and more general insights into control of plant sulfur metabolism.

硫是植物中的一种必需营养素，存在于一系列必需的初级代谢产物以及许多次级化合物中。由于其化学性质，初级代谢产物中的硫通常以还原形式作为硫醇或硫化物掺入，而次级化合物通常含有氧化硫作为有机硫酸盐。我们已经鉴定了拟南芥中腺苷5 ′-磷酸硫酸（APS）激酶的4种亚型，它们对于完成生命周期和合成硫酸化次级产物（如芥子油苷）至关重要。然而，我们的分析留下了几个重要的问题没有回答：（一）APS激酶的氧化还原调节在初级和次级代谢之间控制S分配的作用是什么（ii）为什么APS激酶亚型APK 2单独不能维持拟南芥的生长，和（iii.）APS激酶在不产生芥子油苷的植物物种中的重要性是什么？该提案解决了这些问题，以便获得对植物硫代谢控制的更详细、更全面的见解。

项目成果

Highlighted mechanistic aspects in the chemical biology of reactive sulfur species

• DOI: 10.1111/bph.14551
• 发表时间: 2019-02
• 期刊: British Journal of Pharmacology
• 影响因子: 7.3
• 作者: P. Nagy;G. Schwarz;S. Kopriva