同質・異質倍数体生物の環境適応機構のゲノム基盤

同源和异源多倍体生物环境适应机制的基因组基础

• 批准号: 19F19089
• 负责人: 岩崎 渉
• 金额: $ 1.47万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2019
• 资助国家: 日本
• 起止时间: 2019-04-25 至 2021-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-19F19089/
• 关键词: ゲノム進化; バイオインフォマティクス; genome; evolution

In the second year, we successfully conducted experiments with gradients of increasing harshness with two different kinds of stress, such as fungicide and high salt concentration. As expected, each successive zone required a different mechanism for the fungi to cope with the new environment. The speed of adaptation in this variant became a proxy of how fast the fungi can reorient themselves to new stress with each new zone. We also conducted mixing competition assay into additional culture experiments by making the starting colony a 50:50 mixture of hybrid-diploid pair that share closely related subgenomes. Nanopore sequencing was also conducted to retrieve genomic data. The results are being analyzed in depth.

第二年，我们成功地进行了两种不同胁迫（例如杀菌剂和高盐浓度）的梯度递增实验。正如预期的那样，每个连续的区域都需要真菌不同的机制来应对新环境。这种变体的适应速度代表了真菌在每个新区域重新适应新压力的速度。我们还在额外的培养实验中进行了混合竞争测定，将起始菌落制成具有密切相关亚基因组的杂种二倍体对的 50:50 混合物。还进行了纳米孔测序以检索基因组数据。正在对结果进行深入分析。