Biomedical Applications of High-troughput Raman Flow Cytometry

高通量拉曼流式细胞术的生物医学应用

• 批准号: 19F19805
• 负责人: 合田 圭介
• 金额: $ 1.41万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2019
• 资助国家: 日本
• 起止时间: 2019-11-08 至 2022-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-19F19805/
• 关键词: FT-CARS; Raman; Microalgae; Carbohydrate; Paramylon; Euglena; Starch; Carotenoid; Raman Microalgae; Euglena Starch; Carbohydrate Paramylon

The system pulse-shaping capabilities were improved and a transform-limited pulse was achieved, improving the sensitivity and signal quality for carbohydrates and carotenoids. Heavy ions mutagenesis was performed in Euglena gracilis, identifying hyperaccumulating mutants via FT-CARS paramylon. RNA isolation was done and cDNA analysis was performed. Analysis of this data and manuscript preparation is currently in process. The analysis of starch accumulation in Chromochloris zofingiensis via both FT-CARS and enzymatic analysis were done, and a manuscript is in preparation. Sorting of multiple microalgae species was achieved and findings were recently published in Advanced Materials Technology. Human blood signal was obtained via FT-CARS, likely due to the strong resonant Raman of haemoglobin. However, leukocyte detection is still challenging with the current system sensitivity, and thus the system will require further optimization for clinical applications. An additional application of the FT-CARS system was identified: microplastics detection under flow. Preliminary work was done and has been followed by other researchers after the fellow’s departure.

系统的脉冲整形能力得到改善，实现了变换限制脉冲，提高了碳水化合物和类胡萝卜素的灵敏度和信号质量。重离子诱变在Euglena gracilis中进行，通过FT-CARS副淀粉鉴定超积累突变体。进行RNA分离并进行cDNA分析。目前正在对这些数据进行分析，并正在编写文稿。本文采用傅立叶变换-CARS和酶法分析了新疆绿球藻的淀粉积累过程，研究结果正在准备中。实现了多种微藻物种的分选，研究结果最近发表在Advanced Materials Technology上。通过FT-CARS获得人血液信号，可能是由于血红蛋白的强共振拉曼。然而，白细胞检测在当前系统灵敏度下仍然具有挑战性，因此该系统将需要进一步优化以用于临床应用。确定了FT-CARS系统的另一个应用：流动下的微塑料检测。初步工作已经完成，并在该研究员离开后由其他研究人员继续进行。

项目成果

From coherent quantum photonics to vibrational flow cytometry

从相干量子光子学到振动流式细胞术

• 发表时间: 2021
• 作者: Gala de Pablo;J
• 通讯作者: J

Vibrational flow cytometry on a chip: a label-free tool for metabolic phenotyping.

芯片上的振动流式细胞术：一种用于代谢表型分析的无标记工具。

• 发表时间: 2020
• 作者: Gala de Pablo;J.;Lindley;M.;Hiramatsu;K.;Isozaki A.;Goda K.
• 通讯作者: Goda K.

High-throughput Raman flow cytometry for directed evolution.

用于定向进化的高通量拉曼流式细胞术。

• 发表时间: 2021
• 作者: Gala de Pablo;J
• 通讯作者: J

Vibrational flow cytometry on a chip: a label-free tool for single-cell metabolic phenotyping

芯片上的振动流式细胞术：用于单细胞代谢表型分析的无标记工具

• 发表时间: 2020
• 作者: Gala de Pablo;J.;Lindley;M.;Hiramatsu;K.; Isozaki;A.;Goda;K.
• 通讯作者: K.

Highly sensitive Fourier-transform coherent anti-Stokes Raman scattering spectroscopy via genetic algorithm pulse shaping

• DOI: 10.1364/ol.434054
• 发表时间: 2021-09-01
• 期刊: OPTICS LETTERS
• 影响因子: 3.6
• 作者: Lindley, Matthew;Gala de Pablo, Julia;Goda, Keisuke
• 通讯作者: Goda, Keisuke