Biomedical Applications of High-troughput Raman Flow Cytometry

改善了系统的脉冲整形能力，获得了转换受限脉冲，改善了对碳水化合物和类胡萝卜素的灵敏度和信号质量。利用重离子诱变技术对纤细裸藻进行诱变，通过FT-CARS辅助聚合体鉴定超积累突变体。提取RNA，进行DNA序列分析。目前正在对这些数据进行分析并编写手稿。用FT-CARS和酶分析两种方法对条斑铬绿藻的淀粉积累进行了分析，目前正在准备一份稿件。实现了对多种微藻物种的分选，研究结果最近发表在《先进材料技术》杂志上。人体血液信号是通过FT-CARS获得的，这可能是由于血红蛋白的强共振拉曼效应。然而，在目前的系统灵敏度下，白细胞检测仍然具有挑战性，因此该系统还需要进一步优化才能应用于临床。确定了FT-CARS系统的另一个应用：流动状态下的微塑料检测。在这位研究员离开后，初步工作已经完成，并得到了其他研究人员的关注。

The system pulse-shaping capabilities were improved and a transform-limited pulse was achieved, improving the sensitivity and signal quality for carbohydrates and carotenoids. Heavy ions mutagenesis was performed in Euglena gracilis, identifying hyperaccumulating mutants via FT-CARS paramylon. RNA isolation was done and cDNA analysis was performed. Analysis of this data and manuscript preparation is currently in process. The analysis of starch accumulation in Chromochloris zofingiensis via both FT-CARS and enzymatic analysis were done, and a manuscript is in preparation. Sorting of multiple microalgae species was achieved and findings were recently published in Advanced Materials Technology. Human blood signal was obtained via FT-CARS, likely due to the strong resonant Raman of haemoglobin. However, leukocyte detection is still challenging with the current system sensitivity, and thus the system will require further optimization for clinical applications. An additional application of the FT-CARS system was identified: microplastics detection under flow. Preliminary work was done and has been followed by other researchers after the fellow’s departure.