多様な生体医学応用のためのインテリジェント画像活性化細胞選抜法　の応用展開

智能图像激活细胞选择方法在各种生物医学应用中的应用开发

• 批准号: 19F19716
• 负责人: 合田 圭介
• 金额: $ 1.02万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2019
• 资助国家: 日本
• 起止时间: 2019-07-24 至 2021-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-19F19716/
• 关键词: iIACS; imaging flow cytometry; cells; viscoelastic fluids; 3D focusing

(a) The iIACS machine has been improved, which far surpasses the previous state-of-the-art iIACS machine in system performance, expanding the range of applications and discoveries. Its throughput and sensitivity are 20 times superior to those achieved in previous system.(b) By employing the VIFFI microscope, the iIACS machine has higher imaging performance, holds promise for diverse applications in immunology, microbiology, stem cell biology, cancer biology, pathology, and synthetic biology. The imaging performance of the iIACS machine was validated using various cell types, like the FISH images of the Jurkat cells, imaging and sorting for the budding yeast cells.(c) I have developed a viscoelastic fluid-based 3D focuser, and employed it for single-particle Raman analysis, as well as into the flow module in VIFFI platform, which can enable high-throughput single-cell imaging and analysis with higher stability.

(a)iIACS机器得到了改进，在系统性能上远远超过了以前最先进的iIACS机器，扩大了应用和发现的范围。它的通量和灵敏度是以前系统的上级20倍。(b)通过采用VIFFI显微镜，iIACS机器具有更高的成像性能，有望在免疫学，微生物学，干细胞生物学，癌症生物学，病理学和合成生物学中实现多种应用。使用各种细胞类型验证iIACS机器的成像性能，如Jurkat细胞的FISH图像、芽殖酵母细胞的成像和分选。(c)我开发了一种基于粘弹流体的3D聚焦器，并将其用于单颗粒拉曼分析，并应用于VIFFI平台的流动模块中，可以实现高通量的单细胞成像和分析，具有更高的稳定性。

项目成果

Morphological Indicator for Directed Evolution of Euglena gracilis with a High Heavy Metal Removal Efficiency

具有高重金属去除效率的细小裸藻定向进化的形态指标

• DOI: 10.1021/acs.est.0c05278
• 发表时间: 2021
• 期刊: Environmental Science & Technology
• 影响因子: 11.4
• 作者: Xu Muzhen;Harmon Jeffrey;Yuan Dan;Yan Sheng;Lei Cheng;Hiramatsu Kotaro;Zhou Yuqi;Loo Mun Hong;Hasunuma Tomohisa;Isozaki Akihiro;Goda Keisuke
• 通讯作者: Goda Keisuke

Dean-flow-coupled elasto-inertial particle and cell focusing in symmetric serpentine microchannels

对称蛇形微通道中的迪恩流耦合弹惯性粒子和细胞聚焦

• DOI: 10.1007/s10404-019-2204-3
• 发表时间: 2019-02
• 期刊: Microfluidics and Nanofluidics
• 影响因子: 2.8
• 作者: Yuan Dan;Sluyter Ronald;Zhao Qianbin;Tang Shiyang;Yan Sheng;Yun Guolin;Li Ming;Zhang Jun;Li Weihua
• 通讯作者: Li Weihua

Viscoelastic-fluid-based single-particle analyzer with Fourier-transform coherent Anti-Stokes Raman scattering spectroscopy

基于粘弹性流体的单粒子分析仪，具有傅里叶变换相干反斯托克斯拉曼散射光谱

• 发表时间: 2020
• 作者: Isozaki Akihiro;Hiraki Kei;et al.;Yuan Dan
• 通讯作者: Yuan Dan

Intelligent image-activated cell sorting 2.0

• DOI: 10.1039/d0lc00080a
• 发表时间: 2020-07-07
• 期刊: LAB ON A CHIP
• 影响因子: 6.1
• 作者: Isozaki, Akihiro;Mikami, Hideharu;Goda, Keisuke
• 通讯作者: Goda, Keisuke