Dissecting the genetic basis of root vigor : applications for improving rice for a changing world

剖析根系活力的遗传基础：在不断变化的世界中改良水稻的应用

• 批准号: 19F19709
• 负责人: Wissuwa Matthias
• 金额: $ 1.41万
• 依托单位: Japan International Research Center for Agricultural Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2019
• 资助国家: 日本
• 起止时间: 2019-07-24 至 2021-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-19F19709/
• 关键词: phosphorus efficiency; crossing population; root-vigor; QTL; chromosome 4; P-stress; RNA sequencing

During my JSPS Fellowship, I conducted research focusing on understanding phosphorus(P) efficiency mechanism in rice. My experimental material included two rice breeding parents and their crossing population contrasting in root-vigor related traits and P efficiency in low P soils. I carried out quantitative trait loci (QTL) analysis to determine the underlying genetic basis of the P-efficient root traits. My analysis identified a QTL on chromosome 4 potentially contributing to elongating root length in low P conditions. I also followed up how these contrasting P efficient rice genotypes responded to when P availability was experimentally reduced. My results revealed that the high P-efficient genotype rapidly responded to low P availability by expressing the P-stress related genes.

在JSPS期间，我的研究重点是了解水稻中磷(P)的效率机制。本实验材料采用两个水稻亲本及其杂交群体，在低磷土壤中进行根系活力相关性状和磷效率的比较。通过数量性状位点（QTL）分析，确定磷效根性状的遗传基础。我的分析发现，在低磷条件下，4号染色体上有一个QTL可能导致根长延长。我还跟踪研究了不同磷效率水稻基因型在实验降低磷有效性时的反应。结果表明，高效磷基因型通过表达磷胁迫相关基因，对低磷利用率做出快速响应。