亜鉛欠乏土壌におけるイネの生育を向上させる候補遺伝子の特定

鉴定促进缺锌土壤中水稻生长的候选基因

• 批准号: 14F04732
• 负责人: Wissuwa Matthias
• 金额: $ 1.34万
• 依托单位: Japan International Research Center for Agricultural Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2014
• 资助国家: 日本
• 起止时间: 2014-04-25 至 2017-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14F04732/
• 关键词: crown tissue; Zn deficiency; RNA sequencing; Zn efficient; Zn inefficient; gibberellin; 亜鉛欠乏; 耐性メカニズム; 候補遺伝; レトロトランスポゾンTos17; イネ系統変異体; 土壌の亜鉛欠乏; 候補遺伝子同定; 分子マーカーの開発; 感受性イネ系統; ゲノム; 遺伝子発現データ; マイクロアレーデータ

Studies were followed by a transcriptome analysis of crown tissue of genotypes contrasting for Zn deficiency tolerance. In this study she highlighted the molecular mechanisms triggered by early Zn deficiency in crown tissue, through RNA sequencing of two contrasting groups of several Zn efficient and Zn inefficient genotypes. This method allowed her to 1) identify several novel and well-known Zn transporters involved in Zn retranslocation from the crown to the shoot and roots in response to Zn deficiency; 2) determine that Zn deficiency triggers the conversion of soluble sugars into starch; and 3) detect several candidate genes possibly conferring Zn efficiency, including a monosaccharide transporter, a Zn finger domain-containing protein, a gibberellin stimulated family protein and a plasma membrane polypeptide family protein. This work has been submitted and is under review in the Journal of Experimental Botany:

研究之后进行了对照锌缺乏耐受性基因型的冠组织转录组分析。在这项研究中，她通过对几种锌高效型和锌低效型两组对比的RNA测序，强调了冠组织早期锌缺乏引发的分子机制。这种方法使她能够1)鉴定出几种新的和已知的锌转运蛋白，这些转运蛋白参与锌在锌缺乏时从冠向茎和根的再转运；2)确定锌缺乏触发可溶性糖转化为淀粉；3)检测几个可能具有锌效率的候选基因，包括单糖转运蛋白、含锌指结构域蛋白、赤霉素刺激家族蛋白和质膜多肽家族蛋白。这项工作已经提交并正在《实验植物学杂志》上进行审查：

项目成果

Rice Genotype Differences in Tolerance of Zinc-Deficient Soils: Evidence for the Importance of Root-Induced Changes in the Rhizosphere.

• DOI: 10.3389/fpls.2015.01160
• 发表时间: 2015
• 期刊: Frontiers in plant science
• 影响因子: 5.6
• 作者: Mori A;Kirk GJ;Lee JS;Morete MJ;Nanda AK;Johnson-Beebout SE;Wissuwa M
• 通讯作者: Wissuwa M

Rapid Crown Root Development Confers Tolerance to Zinc Deficiency in Rice.

• DOI: 10.3389/fpls.2016.00428
• 发表时间: 2016
• 期刊: Frontiers in plant science
• 影响因子: 5.6
• 作者: Nanda AK;Wissuwa M
• 通讯作者: Wissuwa M

Patterns of stress response and tolerance based on transcriptome profiling of rice crown tissue under Zn deficiency.

基于缺锌条件下水稻冠部组织转录组分析的胁迫反应和耐受性模式。

• 发表时间: 2016
• 期刊: JXB.
• 作者: Nanda AK;Puyol V;Wissuwa M
• 通讯作者: Wissuwa M

The effects of zinc deficiency on root development in rice

缺锌对水稻根系发育的影响

• 发表时间: 2015
• 作者: Amrit Nanda;Matthias Wissuwa
• 通讯作者: Matthias Wissuwa