Optimising the allocation of phosphorus fractions in rice to improve

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The main experiment of this project, a large-scale hydroponic experiment has been successfully completed. Since the last report, all of the laboratory analysis from this experiment has also been completed. This included analysis of chemical P-fraction concentrations, analysis ofcell-level P concentrations at the Centre for Microscopy, Characterisation and Analysis (CMCA), University of Western Australia, and the preparation and delivery of samples to collaborators at Nara Institute of Science and Technology (NAIST) for analysis of metabolites. The physiological, biomass, total P, and P-fraction data have been combined into one manuscript, currently in preparation, to be submitted to New Phytologist.The cell-level nutrient data has been analysed and will form a second manuscript, currently in preparation. The metabolite data is yet to be received, but we expect it to form a third manuscript. In addition, I collaborated with two other JSPS fellows (Asad Prodhan and Getnet Adem) on a joint experiment assessing short-term changes in metabolites, proteins and gene expression, under different forms of P-stress. This experiment was completed successfully, and we are currently processing samples.In summary, samples from the main experiment have all been analysed or submitted for analysis. Two manuscripts are in preparation, and the third is awaiting results.