Optimising the allocation of phosphorus fractions in rice to improve

优化水稻中磷组分的分配以提高

• 批准号: 18F18711
• 负责人: Wissuwa Matthias
• 金额: $ 1.41万
• 依托单位: Japan International Research Center for Agricultural Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2018
• 资助国家: 日本
• 起止时间: 2018-07-25 至 2021-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-18F18711/
• 关键词: hydroponic experiment; P-fraction concentration; metabolite; PUE; P-fractions; RNA; metabolite analysis; P-allocation; colorimetric method

The main experiment of this project, a large-scale hydroponic experiment has been successfully completed. Since the last report, all of the laboratory analysis from this experiment has also been completed. This included analysis of chemical P-fraction concentrations, analysis ofcell-level P concentrations at the Centre for Microscopy, Characterisation and Analysis (CMCA), University of Western Australia, and the preparation and delivery of samples to collaborators at Nara Institute of Science and Technology (NAIST) for analysis of metabolites. The physiological, biomass, total P, and P-fraction data have been combined into one manuscript, currently in preparation, to be submitted to New Phytologist.The cell-level nutrient data has been analysed and will form a second manuscript, currently in preparation. The metabolite data is yet to be received, but we expect it to form a third manuscript. In addition, I collaborated with two other JSPS fellows (Asad Prodhan and Getnet Adem) on a joint experiment assessing short-term changes in metabolites, proteins and gene expression, under different forms of P-stress. This experiment was completed successfully, and we are currently processing samples.In summary, samples from the main experiment have all been analysed or submitted for analysis. Two manuscripts are in preparation, and the third is awaiting results.

本项目的主要实验--大型水培实验已经顺利完成。自上次报告以来，本次实验的所有实验室分析也已完成。这包括对化学P组分浓度的分析，对西澳大利亚大学显微、特征和分析中心的细胞水平P浓度的分析，以及准备样品并交付给奈良科学技术研究所(NAIST)的合作者进行代谢物分析。生理、生物量、全磷和磷组分的数据已经合并成一份手稿，目前正在准备中，将提交给新植物学家。细胞水平的营养数据已经分析，并将形成第二份手稿，目前正在准备中。代谢物数据尚未收到，但我们预计它将形成第三份手稿。此外，我与JSPS的另外两名研究员(Asad Roghan和Getnet Adem)合作进行了一项联合实验，评估在不同形式的P胁迫下代谢物、蛋白质和基因表达的短期变化。这项实验已经成功完成，我们目前正在处理样品。总而言之，主要实验的样品都已经分析或提交分析。两份手稿正在准备中，第三份正在等待结果。

项目成果

Analysing Cell Level Allocation of Calcium and Phosphorus in Leaves of Proteaceae from South-Western Australia

分析澳大利亚西南部山龙眼科植物叶子中钙和磷的细胞水平分配

• 发表时间: 2019
• 作者: 細見晃司;國澤純;Patrick Hayes
• 通讯作者: Patrick Hayes