Functional characterization of candidate genes for PUE and mining their allelic variants in rice

水稻 PUE 候选基因的功能表征及其等位基因变异挖掘

• 批准号: 18F18703
• 负责人: Wissuwa Matthias
• 金额: $ 1.34万
• 依托单位: Japan International Research Center for Agricultural Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2018
• 资助国家: 日本
• 起止时间: 2018-07-25 至 2021-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-18F18703/
• 关键词: PUE; P-efficient; P-inefficient; rice genotypes; P flux; RNA sequencing; transcriptome analysis; high biomass; metabolic forms of P; fractionation of P; expression; bioinformatics; QPCR; Gene 13; Gene 14; Gene 30; PM-H+ATP; remobilization

To breed rice plants for PUE (Phosphorus use efficiency), understanding the physiological, metabolic, molecular and genetic aspects of PUE is important. In our experiments, P-efficient and P-inefficient rice genotypes were used to understand the above-mentioned traits with different doses of P supply. P-efficient genotypes maintain low concentration of P and P content and high biomass that contributed to the efficiency of the rice plant in phosphorus utilization. In addition, the P-efficient rice genotypes showed P flux from old to young leaves. In another experiment, the P-efficient genotypes showed reallocation of P from shoot to root where sampling was done at six time intervals in approx. 2 days gap.The fractionation of P into organic, nucleic acid, inorganic and metabolic components of P was studied in collaboration with Dr. Patrick Hayes (JSPS Fellow (P18711). Leaf cell-specific P-allocation was also another component looked at in this experiment. Similarly, transcriptome analysis using RNA sequencing was done for P-efficient and P-inefficient rice genotypes under different P supply. This experiment was done in collaboration with Dr. Asad Prodhan (JSPS Fellow (P19709)). These parts of the experiment will be reported by the above 2 JSPS fellows to avoid redundancy.

为了培育磷利用效率(PUE)的水稻植株，了解PUE的生理、代谢、分子和遗传方面是很重要的。本试验以磷高效和磷低效两个水稻品种为材料，研究了不同施磷量对上述性状的影响。磷高效型水稻植株维持低浓度的磷和磷含量和较高的生物量，这有助于提高植株对磷的利用效率。此外，磷高效水稻品种表现出从老叶到幼叶的磷素通量。在另一项实验中，磷高效的基因型表现出磷从地上部到根的重新分配，在大约6个时间间隔进行采样。与帕特里克·海斯博士(JSPS For(P18711))合作研究了磷的有机、核酸、无机和代谢组分。叶细胞特有的磷分配也是这项实验中观察到的另一个组成部分。同样，利用RNA测序技术对不同磷供应条件下磷高效和磷低效水稻的转录组进行了分析。这项实验是与阿萨德·普罗汉博士(JSPS For(P19709))合作完成的。实验的这些部分将由上述两名JSPS研究员报告，以避免冗余。