The Role of Small Single Transmembrane Helix Proteins in Cyanobacteria

小单跨膜螺旋蛋白在蓝藻中的作用

• 批准号: 379644404
• 负责人: Professor Dr. Marc Nowaczyk
• 金额: --
• 依托单位: Lehrstuhl Biochemie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/379644404?language=en
• 关键词: Role; Small; Single; Transmembrane; Helix

Membrane protein complexes play a crucial role in bioenergetics, particularly in photosynthetic organisms like plants, algae and cyanobacteria, as they are responsible for the light-to-chemical energy conversion in the primary reactions of photosynthesis. All of them – as far as being analyzed – contain up to 10 small (<50 AA) proteins with at least one single transmembrane helix (sTMH proteins). However, the discovery and further characterization of sTMH proteins is severely hampered by their small size and often extreme hydrophobicity. We will use two different approaches to shed light on the role of sTMH proteins in cyanobacteria. In a top-down approach we will use our well-established pipeline for the purification of affinity-tagged membrane protein complexes and subsequent in-depth mass spectrometry analysis. For a bottom-up approach, we have identified potential sTMH candidates through an in silico screening, which will be further analyzed by biochemical and biophysical methods. Final goal will be to gain comprehensive knowledge about the molecular function of sTMH proteins in cyanobacteria.

膜蛋白复合物在生物能学中起着至关重要的作用，尤其是在植物，藻类和蓝细菌等光合生物中，因为它们负责光合作用的主要反应的光到化学能量转化。所有这些（就被分析而言）包含至少一个单个跨膜螺旋（STMH蛋白）的最多10个小（<50 aa）蛋白。然而，STMH蛋白的发现和进一步表征由于其小的尺寸和经常极端疏水而严重阻碍了STMH蛋白。我们将使用两种不同的方法来阐明STMH蛋白在蓝细菌中的作用。在自上而下的方法中，我们将使用我们建立的仪器来纯化亲和力标记的膜蛋白复合物以及随后的深入质谱分析。对于自下而上的方法，我们通过硅筛选确定了潜在的STMH候选物，这将通过生化和生物物理方法进一步分析。最终目标是获得有关蓝细菌中STMH蛋白的分子功能的全面知识。