The evolution of regenerative capacities in tetrapod limbs - a deep time perspective combining extant and fossil data

四足动物四肢再生能力的进化——结合现存数据和化石数据的深度时间视角

• 批准号: 386259701
• 负责人: Professorin Dr. Nadia Belinda Fröbisch
• 金额: --
• 依托单位: Museum für Naturkunde - Leibniz-Institut für Evolutions- und Biodiversitätsforschung (MfN)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/386259701?language=en
• 关键词: evolution; regenerative; capacities; tetrapod; limbs

Salamanders are extraordinary among tetrapods because of their extremely high regenerative capacities that includes parts of internal organs, lenses of the eye, and full limb and tail regeneration. Particularly limb regeneration has been subject to intensive research and great advances have been made in the past decades, especially with respect to the molecular control of limb regeneration. The vast majority of studies on limb regeneration have used the Mexican axolotl Ambystoma mexicanum, a neotenic ambystomatid salamander, as model organism in laboratory experiments. In contrast, very little is known on the diversity of regenerative capacities within the morphologically and ecologically diverse clade of salamanders and how regeneration may differ in salamanders with life history patterns different from the axolotl. However, together with the fossil record this data can provide important insights into the evolutionary history of regenerative capacities in the tetrapod limb and aid in identifying shared versus derived pathways in regeneration. This proposal takes an integrative approach combining paleohistological data from the fossil stem lineage of modern amphibians, histological data from extant salamanders, and comparative transcriptome data to gain a multifaceted picture of the evolutionary history of limb regeneration in tetrapods. Limb regeneration will be investigated histologically and with CT imaging techniques in selected salamander taxa to assess how speed and accuracy of limb regeneration vary along ontogenetic trajectories within taxa and between taxa with different life history pathways, including neoteny, metamorphosis and direct development. A paleohistological investigation of regenerated limbs in the Permian dissorophoid Micromelerpeton will form the basis for comparison with extant salamanders with respect to tissue level organization and regenerative anomalies. Moreover, comparative transcriptome data will be used to investigate the molecular signatures of limb regeneration in larval, neotenic, and metamorphosing salamanders, as well as lungfish and frogs. The combination of the data of these approaches will provide a novel basis for our understanding of the evolutionary history of limb regeneration in tetrapods, as well as highlight future research avenues on the impact of ontogeny and life history parameters in regeneration, and potentially shifting roles of certain molecular signals (e.g. stem cell signals) in regeneration along these trajectories.

蝾螈在四足动物中是非凡的，因为它们具有极高的再生能力，包括部分内脏、眼睛的晶状体、完整的肢体和尾巴的再生。特别是肢体再生在过去几十年里得到了广泛的研究，并取得了很大的进展，特别是在肢体再生的分子控制方面。绝大多数关于肢体再生的研究都是在实验室实验中使用墨西哥蝾螈Ambystoma mexicanum作为模式生物，Ambystoma mexicanum是一种新生的ambystomid蝾螈。相比之下，在形态和生态多样性的蝾螈分支中，再生能力的多样性以及与蝾螈不同生活史模式的蝾螈的再生能力如何不同，人们知之甚少。然而，结合化石记录，这些数据可以为四足动物肢体再生能力的进化史提供重要的见解，并有助于识别再生中的共享途径和衍生途径。本研究采用综合的方法，结合来自现代两栖动物化石茎系的古组织学数据、来自现存蝾螈的组织学数据和比较转录组数据，以获得四足动物肢体再生进化史的多面图。本文将对蝾螈的肢体再生进行组织学研究和CT成像技术研究，以评估不同类群的肢体再生速度和准确性在类群内部和不同生活史路径的类群之间（包括幼期、蜕变和直接发育）的变化。对二叠纪非sorophoid Micromelerpeton再生肢体的古组织学研究将形成与现存蝾螈在组织水平组织和再生异常方面进行比较的基础。此外，比较转录组数据将用于研究幼体、新生期和变态蝾螈、肺鱼和青蛙肢体再生的分子特征。这些方法的数据结合将为我们理解四足动物肢体再生的进化史提供新的基础，并为未来的研究途径提供重点，包括个体发生和生活史参数对再生的影响，以及某些分子信号（如干细胞信号）在这些轨迹上的再生中的潜在转变作用。

项目成果

Deep evolutionary origin of limb and fin regeneration

• DOI: 10.1073/pnas.1900475116
• 发表时间: 2019-07-23
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Darnet, Sylvain;Dragalzew, Aline C.;Schneider, Igor
• 通讯作者: Schneider, Igor

Salamander-like tail regeneration in the West African lungfish

• DOI: 10.1098/rspb.2019.2939
• 发表时间: 2020-02
• 期刊: bioRxiv
• 作者: K. M. Verissimo;Louise N. Perez;Aline C. Dragalzew;G. Senevirathne;Sylvain Darnet;W. R. Mendes;Ciro Ariel dos Santos Neves;Erika Monteiro dos Santos;C. N. de Sousa Moraes;Ahmed Elewa-;N. Shubin;N. B. Froebisch;J. de Freitas Sousa;I. Schneider

A Morphological and Histological Investigation of Imperfect Lungfish Fin Regeneration

肺鱼鳍再生不完善的形态学和组织学研究

• DOI: 10.3389/fevo.2021.784828
• 发表时间: 2021
• 作者: Vivien;Igor Schneider;Nadia B. Fröbisch
• 通讯作者: Nadia B. Fröbisch