Transcriptional control of cellular reprogramming during the symbiotic interaction of Medicago truncatula with arbuscular mycorrhizal fungi

蒺藜苜蓿与丛枝菌根真菌共生相互作用过程中细胞重编程的转录控制

• 批准号: 38723928
• 负责人: Professor Dr. Helge Küster
• 金额: --
• 依托单位: Abteilung IV: Pflanzengenomforschung
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/38723928?language=en
• 关键词: Transcriptional; control; cellular; reprogramming; during

Arbuscular mycorrhizae (AM) are the most widespread beneficial plant-microbe interactions on Earth. Due to the availability of comprehensive sequence information for the model legume Medicago truncatula and the model AM fungus Glomus intraradices, genome-wide expression profiling experiments are possible. To understand AM-related transcriptional reprogramming, we investigate three levels of signaling: rhizodermal cells perceiving diffusible Myc-factors, root cells infected by mycorrhizal fungi, and cortical cells establishing functional arbuscules. Since Myc-factors emerged as lipochitooligosaccharides similar to rhizobial Nod-factors, we will use symbiotic mutants to discriminate Nod- and Myc-factor signaling, in order to elucidate Myc-factor specific responses. Via laser microdissection, cell-specific gene expression during early and late AM stages is investigated. Based on these experiments, in situ markers for Myc-factor activity can be developed, and promoter motifs related to the control of gene expression in arbuscule-containing cells will be studied further. Reverse genetics continues to be performed for candidate genes selected by transcriptome profiling to investigate their role during early and late cellular reprogramming. Candidate genes identified so far are related to early AM signaling upstream and downstream of the calcium/calmodulin-dependent protein kinase DMI3, or are expressed in arbuscule-containing cells. In total, 12 Tnt1 insertion mutant lines are available that in addition to RNAi approaches can now be used for detailed functional studies.

丛枝菌根（AM）是地球上最广泛的有益植物-微生物相互作用。由于模型豆科植物苜蓿（Medicago truncatula）和模型AM真菌Glomus intraradices的完整序列信息的可用性，使全基因组表达谱实验成为可能。为了理解am相关的转录重编程，我们研究了三个水平的信号传导：根真皮细胞感知扩散性myc因子，根细胞被菌根真菌感染，皮质细胞建立功能性丛枝。由于myc -因子以脂壳寡糖的形式出现，类似于根瘤菌的Nod-因子，我们将使用共生突变体来区分Nod-和myc -因子信号，以阐明myc -因子的特异性反应。通过激光显微解剖，研究AM早期和晚期细胞特异性基因的表达。基于这些实验，可以开发myc因子活性的原位标记，并进一步研究与丛枝细胞中基因表达控制相关的启动子基序。反向遗传学继续对转录组分析选择的候选基因进行研究，以研究它们在早期和晚期细胞重编程中的作用。目前发现的候选基因与钙/钙调素依赖性蛋白激酶DMI3上游和下游的早期AM信号有关，或者在含束细胞中表达。总共有12个Tnt1插入突变系可用，除了RNAi方法外，现在可以用于详细的功能研究。